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Title: Utilization of enzymatically hydrolyzed wood hemicelluloses by microorganisms for production of liquid fuels

Abstract

Hemicellulose-derived sugars were obtained from a variety of pretreated wood substrates such as water-soluble fractions from steam-exploded aspen, solvent-extracted aspen, and commercial xylan. These fractions were enzymatically hydrolyzed by commercial enzyme preparations and by the culture filtrates of eight highly cellulolytic fungi. The sugars released were assayed by high-pressure liquid chromatography. Over 30% of the hemicellulose fractions, at a 10% substrate concentration, could be hydrolyzed to monosaccharides. These hemicellulose hydrolysates were used as the substrates for growth of Clostridium acetobutylicum and Klebsiella pneumoniae. Comparatively low butanol values were obtained with C. acetobutylicum, although over 50% of the hemicellulose fraction, at a 1% substrate concentration, could be converted to 2,3-butanediol, ethanol, and acetic acid by K. pneumoniae. (Refs. 25).

Authors:
; ; ; ;
Publication Date:
Research Org.:
Biotechnology and Chemistry Groups, Eastern Lab, Forintek Canada Corp, Ottawa, Ontario, Canada K1G 3Z5
OSTI Identifier:
5676554
Alternate Identifier(s):
OSTI ID: 5676554
Resource Type:
Journal Article
Resource Relation:
Journal Name: Appl. Environ. Microbiol.; (United States); Journal Volume: 45:1
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ACETIC ACID; PRODUCTION; BUTANEDIOLS; BUTANOLS; ETHANOL; HEMICELLULOSE; ENZYMATIC HYDROLYSIS; CLOSTRIDIUM ACETOBUTYLICUM; FERMENTATION; GROWTH; KLEBSIELLA; MONOSACCHARIDES; SACCHARIFICATION; WOOD; ALCOHOLS; BACTERIA; BIOCONVERSION; CARBOHYDRATES; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; CLOSTRIDIUM; DECOMPOSITION; GLYCOLS; HYDROLYSIS; HYDROXY COMPOUNDS; LYSIS; METHANOGENIC BACTERIA; MICROORGANISMS; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS 090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989); 550700 -- Microbiology; 140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)

Citation Formats

Saddler, J.N., Yu, E.K.C., Mes-Hartree, M., Levitin, N., and Brownell, H.. Utilization of enzymatically hydrolyzed wood hemicelluloses by microorganisms for production of liquid fuels. United States: N. p., 1983. Web.
Saddler, J.N., Yu, E.K.C., Mes-Hartree, M., Levitin, N., & Brownell, H.. Utilization of enzymatically hydrolyzed wood hemicelluloses by microorganisms for production of liquid fuels. United States.
Saddler, J.N., Yu, E.K.C., Mes-Hartree, M., Levitin, N., and Brownell, H.. Sat . "Utilization of enzymatically hydrolyzed wood hemicelluloses by microorganisms for production of liquid fuels". United States. doi:.
@article{osti_5676554,
title = {Utilization of enzymatically hydrolyzed wood hemicelluloses by microorganisms for production of liquid fuels},
author = {Saddler, J.N. and Yu, E.K.C. and Mes-Hartree, M. and Levitin, N. and Brownell, H.},
abstractNote = {Hemicellulose-derived sugars were obtained from a variety of pretreated wood substrates such as water-soluble fractions from steam-exploded aspen, solvent-extracted aspen, and commercial xylan. These fractions were enzymatically hydrolyzed by commercial enzyme preparations and by the culture filtrates of eight highly cellulolytic fungi. The sugars released were assayed by high-pressure liquid chromatography. Over 30% of the hemicellulose fractions, at a 10% substrate concentration, could be hydrolyzed to monosaccharides. These hemicellulose hydrolysates were used as the substrates for growth of Clostridium acetobutylicum and Klebsiella pneumoniae. Comparatively low butanol values were obtained with C. acetobutylicum, although over 50% of the hemicellulose fraction, at a 1% substrate concentration, could be converted to 2,3-butanediol, ethanol, and acetic acid by K. pneumoniae. (Refs. 25).},
doi = {},
journal = {Appl. Environ. Microbiol.; (United States)},
number = ,
volume = 45:1,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1983},
month = {Sat Jan 01 00:00:00 EST 1983}
}
  • Hemicellulose-derived sugars were obtained from a variety of pretreated wood substrates such as water-soluable fractions from steam-exploded aspen, solvent-extracted aspen, and commercial zylan. These fractions were enzymatically hydrolyzed by commercial enzyme preparations and by the culture filtrates of eight highly celludolytic fungi. The sugars released were assayed by high-pressure liquid chromatography. Over 30% of the hemicellulose fractions, at a 10% substrate concentration, could be hydrolyzed to monosaccharides. These hemicellulose hydrolysates were used as the substrates for growth of Clostridium acetobutylicum and Klebsiella pneumoniae. Comparatively low butanol values were obtained with C. acetobutylicum, although over 50% of the hemicellulose fraction, atmore » a 1% substrate concentration, could be converted to 2,3-butanediol, ethanol, and acetic acid by K. pneumoniae.« less
  • Solid-liquid separation of intermediate process slurries is required in some process configurations for the conversion of lignocellulosic biomass to transportation fuels. Thermochemically pretreated and enzymatically hydrolyzed corn stover slurries have proven difficult to filter due to formation of very low permeability cakes that are rich in lignin. Treatment of two different slurries with polyelectrolyte flocculant was demonstrated to increase mean particle size and filterability. Filtration flux was greatly improved, and thus scaled filter unit capacity was increased approximately 40-fold compared with unflocculated slurry. Although additional costs were accrued using polyelectrolyte, techno-economic analysis revealed that the increase in filter capacity significantlymore » reduced overall production costs. Fuel production cost at 95% sugar recovery was reduced by $1.35 US per gallon gasoline equivalent for dilute-acid pretreated and enzymatically hydrolyzed slurries and $3.40 for slurries produced using an additional alkaline de-acetylation preprocessing step that is even more difficult to natively filter.« less
  • The authors extended their previous investigations of enzymatic hydrolysis of polysaccharides in orange peel by commercial cellulase and pectinase enzymes to higher, more practical concentrations of orange peel solids. High yields of saccharification could be maintained even at substrate concentrations as high as 22-23%, but the rates of solubilization and saccarification decreased 2-3-fold. They also tested the fermentability of these hydrolysates by the yeast Saccharomyces cerevisiae, which revealed the presence of inhibitory compounds. These compounds could be removed by the filtration of hydrolyzed peel. Successful fermentations of filtered hydrolysates were achieved after pH adjustment with calcium carbonate. 27 refs., 6more » figs., 1 tab.« less