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Utilization of enzymatically hydrolyzed wood hemicelluloses by microorganisms for production of liquid

Journal Article · · Appl. Environ. Microbiol.; (United States)
OSTI ID:5495438
; ; ; ;
Hemicellulose-derived sugars were obtained from a variety of pretreated wood substrates such as water-soluable fractions from steam-exploded aspen, solvent-extracted aspen, and commercial zylan. These fractions were enzymatically hydrolyzed by commercial enzyme preparations and by the culture filtrates of eight highly celludolytic fungi. The sugars released were assayed by high-pressure liquid chromatography. Over 30% of the hemicellulose fractions, at a 10% substrate concentration, could be hydrolyzed to monosaccharides. These hemicellulose hydrolysates were used as the substrates for growth of Clostridium acetobutylicum and Klebsiella pneumoniae. Comparatively low butanol values were obtained with C. acetobutylicum, although over 50% of the hemicellulose fraction, at a 1% substrate concentration, could be converted to 2,3-butanediol, ethanol, and acetic acid by K. pneumoniae.
Research Organization:
Forintek Canada Corp., Ottawa, Ontario
OSTI ID:
5495438
Journal Information:
Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 45:1; ISSN AEMID
Country of Publication:
United States
Language:
English

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Related Subjects

09 BIOMASS FUELS
090222 -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
ACETIC ACID
ALCOHOLS
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
BUTANEDIOLS
BUTANOLS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELLULOLYTIC ACTIVITY
CHEMICAL REACTIONS
CLOSTRIDIUM
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ETHANOL
GLYCOLS
HEMICELLULOSE
HYDROLYSIS
HYDROXY COMPOUNDS
KLEBSIELLA
LYSIS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
WOOD