Enzymatic hydrolysis of various pretreated lignocellulosic substrates and the fermentation of the liberated sugars to ethanol and butanediol

Conference · Sat Jan 01 04:00:00 EST 1983 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:7077505

Saddler, J N; Mes-Hartree, M; Yu, E K.C.; Brownell, H H

Aspen wood and wheat straw were pretreated by exposure to steam at elevated temperatures. Chemical analysis of the substrates revealed that steam explosion differentially decomposed the pentosan component while leaving the glucan portion relatively unchanged. The pretreated residues could be used as substrates for growth of Trichoderma reesei C30 and T. harzianum E58. The cellulase activities detected were in some cases three times as high as those found when Solka Floc was used as the substrate. Culture filtrates of T. harzianum E58 could efficiently hydrolyze the hemicellulose-rich water-soluble fractions. This material was fermented by Klebsiella pneumoniae with 0.4-0.5 g of 2,3-butanediol produced per gram of sugar utilized. Once the steam-exploded residues had been water and alkali extracted, the enzymatically hydrolyzed substrates were readily fermented by Saccharomyces cerevisiae or Zymononas mobilis with values as high as 2% (w/v) ethanol obtained from 5% steam-exploded wood fractions. 30 references, 2 figures, 8 tables.

• Research Organization: Forintek Canada Corp., Ottawa, Ontario
• OSTI ID: 7077505
• Report Number(s): CONF-830567-
• Conference Information: Journal Name: Biotechnol. Bioeng. Symp.; (United States) Journal Volume: 13
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ASPENS
BACTERIA
BUTANEDIOLS
CARBOHYDRATES
CELLULASE
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
ETHANOL
FUNGI
GLYCOLS
GLYCOSYL HYDROLASES
HEAT TREATMENTS
HEMICELLULOSE
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
KLEBSIELLA
LYSIS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PRODUCTION
SACCHARIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SOLVOLYSIS
STEAM
STRAW
SUBSTRATES
TREES
TRICHODERMA
TRICHODERMA VIRIDE
WOOD
YEASTS
ZYMOMONAS MOBILIS