Enzymatic hydrolysis of cellulose and various pretreated wood fractions

Journal Article · Tue Jun 01 04:00:00 EDT 1982 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260240611· OSTI ID:6747911

Saddler, J N; Brownell, H H; Clermont, L P; Levitin, N

Three strains of Trichoderma-Trichoderma reesei C30, Trichoderma reesei QM9414, and Trichoderma species E58-were used to study the enzymatic hydrolysis of pretreated wood substrates. Each of the culture filtrates was incubated with a variety of commercially prepared cellulose substrates and pretreated wood substrates. Solka floc was the most easily degraded commercial cellulose. The enzyme accessibility of steam-exploded samples which has been alkali extracted and then stored wet decreased with the duration of the steam treatment. Air drying reduced the extent of hydrolysis of all the samples but had a greater effect on the samples which had previously shown the greatest hydrolysis. Mild pulping using 2% chlorite increased the enzymatic hydrolysis of all the samples. Steam explosion was shown to be an excellent pretreatment method for aspen wood and was much superior to dilute nitric acid pretreatment. The results indicate that the distribution of the lignin as well as the surface area of the cellulosic substrate are important features in enzymatic hydrolysis. (Refs 17).

• Research Organization: Biotechnology and Chemistry Groups, Eastern Forest Products Lab, Forintek Canada Corp, 800 Montreal Road, Ottawa, Ontario, Canada K1G 325
• OSTI ID: 6747911
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 24:6; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
ASPENS
CARBOHYDRATES
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
FUNGI
HYDROLYSIS
LIGNIN
LYSIS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
STEAM
SUBSTRATES
TREES
TRICHODERMA
TRICHODERMA VIRIDE
WOOD