Butanediol production from cellulose and hemicellulose by Klebsiella pneumoniae grown in sequential coculture with Trichoderma harzianum
Journal Article
·
· Appl. Environ. Microbiol.; (United States)
OSTI ID:5666949
The bioconverison of cellulose and hemicellulose substrates to 2,3-butanediol by a sequential coculture approach was investigated with the cellulolytic fungus Trichoderma harzianum E58 and the fermentative bacterium Klebsiella pneumoniae. Vogel medium optimal for the production of the cellulolytic and xylanolytic enzymes of the fungus was found to be inhibitory to butanediol fermentation. This inhibition appeared to be due to a synergistic effect of various ingredients, particularly the salts, present in the fungal medium. The removal or replacement of such ingredients from Vogel medium led to the relief of fermentation inhibition, but the treatments also resulted in a significant decrease in fungal enzyme production. Resting cells of K. pneumoniae could be used for butanediol production in the fungal medium, indicating that the inhibitory effect on solvent production under such conditions was due to the indirect result of growing inhibition of the bacterial cells. The resting-cell approach could be combined with a fed-batch system for the direct conversion of 8 to 10% (wt/vol) of Solka-Floc or aspenwood xylan to butanediol at over 30% of the theoretical conversion efficiencies.
- Research Organization:
- Forintek Canada Corp., Ottawa
- OSTI ID:
- 5666949
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 50:4; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Journal Article
·
Tue Sep 01 00:00:00 EDT 1987
· Biotechnol. Bioeng.; (United States)
·
OSTI ID:5493471
Combined enzymatic hydrolysis and fermentation approach to butanediol production from cellulose and hemicellulose carbohydrates of wood and agricultural residues
Conference
·
Sat Dec 31 23:00:00 EST 1983
· Biotechnol. Bioeng. Symp.; (United States)
·
OSTI ID:6151120
Enzymatic hydrolysis of various pretreated lignocellulosic substrates and the fermentation of the liberated sugars to ethanol and butanediol
Conference
·
Fri Dec 31 23:00:00 EST 1982
· Biotechnol. Bioeng. Symp.; (United States)
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OSTI ID:7077505
Related Subjects
09 BIOMASS FUELS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
ALCOHOLS
BACTERIA
BATCH CULTURE
BIOCONVERSION
BIOSYNTHESIS
BUTANEDIOLS
CARBOHYDRATES
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
FUNGI
GLYCOLS
HEMICELLULOSE
HYDROLYSIS
HYDROXY COMPOUNDS
INHIBITION
KLEBSIELLA
LYSIS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
TRICHODERMA
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
ALCOHOLS
BACTERIA
BATCH CULTURE
BIOCONVERSION
BIOSYNTHESIS
BUTANEDIOLS
CARBOHYDRATES
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
FUNGI
GLYCOLS
HEMICELLULOSE
HYDROLYSIS
HYDROXY COMPOUNDS
INHIBITION
KLEBSIELLA
LYSIS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
TRICHODERMA