Subpopulation of human helper and suppressor T lymphocytes
Mitogen driven differentiation of normal human mononuclear cells is a well-established model for the study of antibody synthesis in man. In certain rare individuals who are clinically normal, unfractionated mononuclear cells or a mixture of purified B plus T lymphocytes differentiate into immunoglobulin producing cells in response to purified protein derivative of tuberculin (PPD) but not in response to pokeweed mitogen (PWM). To evaluate this observation we have irradiated T cells from such individuals to eliminate naturally occurring suppressor T cell activity and then added the irradiated T cells back to autologous B cells before culture. The B cells then responded to PWM. The original PPD responses of cells from these individuals were now significantly reduced. Although, there was no difference between PWM nonresponders and responders in the number of OKT-8 positive cells, elimination of OKT-8 positive cells in the PWM nonresponders with OKT-8 monoclonal antibody and complement resulted in a significantly increased response to PWM. This study indicates that there are suppressor T cells which specifically inhibit B cell response to PWM without affecting the PPD response. These results also show that the helper T cells involved in the PWM response are radioresistant and those involved in the PPD response are radiosensitive.
- Research Organization:
- Division of Hematology/Oncology, Mount Sinai Hospital Medical Center, Chicago, IL
- OSTI ID:
- 5671563
- Journal Information:
- Hum. Immunol.; (United States), Journal Name: Hum. Immunol.; (United States) Vol. 7:3; ISSN HUIMD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIBODIES
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MITOGENS
MONOCYTES
RADIATION EFFECTS
RADIOSENSITIVITY
SOMATIC CELLS
SYNTHESIS
TUBERCULIN