Differentiation of human B lymphocyte subpopulations induced by an alloreactive helper T-cell clone

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · J. Clin. Immunol.; (United States)

DOI:https://doi.org/10.1007/BF00916556· OSTI ID:6755769

Anderson, S J; Hummell, D S; Lawton, A R

We have used cloned alloreactive helper T cells to determine if direct T cell-B cell interaction can induce differentiation of human peripheral blood B cells which do not respond to pokeweed mitogen (PWM). T-cell clone 2F8 was derived from a one-way mixed lymphocyte reaction. 2F8 cells are T3+T4+T8-IL-2R+ and proliferate in response to irradiated stimulator cells, but not autologous cells, in the absence of exogenous interleukin-2. 2F8 cells provide allospecific help for polyclonal proliferation and differentiation of B cells in the absence of any other stimulus. The magnitude of this response is comparable to that of the response of the same B cells to PWM and fresh autologous T cells. 2F8 cells could also provide nonspecific help for unrelated donor B cells in the presence of PWM, with no requirement for costimulation by irradiated stimulator cells. Allospecific stimulation of B cells was completely inhibited by antibodies to class II major histocompatibility complex (MHC) framework determinants and was abrogated by 1000-rad irradiation. Cloned 2F8 T cells stimulated differentiation of both small, high-density B cells and larger B cells, generating up to 30% plasma cells with either fraction. B cells forming rosettes with mouse erythrocytes were also induced to differentiate by the helper T cell clone. As found previously, neither small, high-density B cells nor mouse rosette+ B cells responded well to PWM. Direct interaction with allospecific T cells induces differentiation of a broader spectrum of B cells than soluble growth and differentiation factors in conjunction with polyclonal activators such as PWM and protein A containing staphylococci.

• Research Organization: Vanderbilt Univ. School of Medicine, Nashville, TN (USA)
• OSTI ID: 6755769
• Journal Information: J. Clin. Immunol.; (United States), Journal Name: J. Clin. Immunol.; (United States) Vol. 8:4; ISSN JCIMD
• Country of Publication: United States
• Language: English

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