The influence of autologous cell interactions on spontaneous and pokeweed mitogen-induced immunoglobulin production

Journal Article · Wed May 01 04:00:00 EDT 1985 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(85)90023-1· OSTI ID:5095636

Beale, M G

Both helper- and suppressor-T-cell activities are generated in the autologous mixed lymphocyte reaction and in pokeweed mitogen (PWM)-stimulated cultures. The addition of low numbers of irradiated non-T cells enhance while high numbers suppress spontaneous and PWM-stimulated IgG synthesis by autologous cells. Monocytes are the principal inducers of suppression and exert their influence within the first 24 hr of culture. Suppression in association with PWM stimulation is nonspecific in nature, T-cell mediated, partially radiosensitive, and resistant to hydrocortisone. Neither indomethacin nor dibutyryl cyclic AMP reverses monocyte-related suppression. These findings suggest that the outcome of in vitro Ig synthesis assays is critically dependent upon monocyte-T-cell interaction.

• OSTI ID: 5095636
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 2; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
ANTIBODY FORMATION
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
FUNCTIONS
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
KETONES
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MITOGENS
MONOCYTES
ORGANIC COMPOUNDS
PHYSIOLOGY
PREGNANES
SOMATIC CELLS
STEROID HORMONES
STEROIDS