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Cellular interactions of human T cell subsets defined by monoclonal antibodies in regulating B cell differentiation: a comparative study in Nocardia water-soluble mitogen- and pokeweed mitogen-stimulated culture systems

Journal Article · · J. Immunol.; (United States)
OSTI ID:5579479
; ; ; ; ;

Two distinct human T cell subsets, OKT4/sup +/ cellrich and OKT8/sup +/ cellrich populations, were negatively selected with reasonable purity by C-mediated cytolysis with the use of monoclonal OKT4 and OKT8 antibodies. B cells were purified by rigorous depletion of E rosetting cells. Purified B cells responded to pokeweed mitogen (PWM) to yield a negligible number of immunoglobulin-producing cells (lg-PC), which were identified by a direct immunofluorescence method, after 7 days of culture, and to Nocardia water-soluble mitogen (NWSM) with the generation of a few, but a significant number of, lg-PC. Helper function of each T cell subset was measured as the ability of added T cells to restore the generation of lg-PC by B cells after 7 days of culture with NWSM or PWM. Results indicate that although NWSM by itself did not activate suppressor T cells to become effector cells, OKT8/sup +/ cells in the NWSM-driven system were able to exert suppressor effect for B cell differentiation only when used in combination with PWM-prestimulated OKT4/sup +/ cells. PWM-prestimulated OKT4/sup +/ cells had a pivotal role for the expression of suppressor activity by OKT8/sup +/ cells in this system, and the ability of these OKT4/sup +/ cells to interact with OKT8/sup +/ cells appeared to be radiosensitive.

Research Organization:
Kanazawa Univ., Japan
OSTI ID:
5579479
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 128:2; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIFFERENTIATION
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
GLOBULINS
IMMUNOGLOBULINS
IMMUNOSUPPRESSION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MINUTES LIVING RADIOISOTOPES
MITOGENS
MONOCLONAL ANTIBODIES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PROTEINS
RADIATION EFFECTS
RADIOISOTOPES
RADIOSENSITIVITY
SOMATIC CELLS
YEARS LIVING RADIOISOTOPES