In vivo production of macrophage migration inhibition and stimulation factors during the inductive phase of the alloimmune response
This paper offers a study of the production of macrophage migration inhibition factor (MIF), and also of the alternative macrophage migration stimulation factor (MSF), in vivo. Mice were injected with mouse spleen cells, irradiated with a dose of 1500 rads. The animals were divided into three groups, two of which were injected for a second time with irradiated mouse spleen cells. Samples of all fractions obtained by electrophoresis of sera of unimmunized mice had no significant effect of macrophage migration, while unfractionated sera of immunized mice obtained after a second injection of alloantigen as a rule stimulated macrophage migration. The results are evidence that T cells may function in vivo during the period before development of the antigen-specific proliferative response of T cells. The technique used to approach the problem, described in this study, can be used for preparative isolation of purified MIF and MSF without contamination by embryonic calf serum proteins which are usually present in culture in vitro.
- Research Organization:
- All-Union Oncologic Research Center, Moscow, USSR
- OSTI ID:
- 5665044
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 102:7; Other Information: Translated from Byulleten' Ehksperimental'noj Biologii i Meditsiny; 102: No. 7, 66-68(Jul 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
MACROPHAGES
IMMUNE REACTIONS
MIGRATION
SPLEEN CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ANTIGENS
BIOSYNTHESIS
ELECTROPHORESIS
EXTERNAL IRRADIATION
IMMUNE SERUMS
IMMUNOGLOBULINS
IN VIVO
INHIBITION
INTRAVENOUS INJECTION
LYMPHOCYTES
MICE
RESPONSE MODIFYING FACTORS
STIMULATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
GLOBULINS
INJECTION
INTAKE
IRRADIATION
LEUKOCYTES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550300 - Cytology