Regulation of cell-mediated cytotoxicity. II. Synergism of two types of thymus dependent cells in vivo. [Mice, gamma radiation]

Journal Article · Tue Jul 01 00:00:00 EDT 1975 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(75)90038-6· OSTI ID:7190226

Sabbadini, E

Sublethal (500 rads) doses of radiation given to mice before the intravenous injection of allogeneic spleen cells induced the development of an increased cell-mediated cytotoxicity (CMC) of the recipients' spleen cells. The effector cells from the irradiated animals were shown to carry the theta alloantigenic marker and to be capable of transferring adoptive immunity in vivo. On the other hand, irradiation of mice with the same dose before the administration of skin or tumor allografts induced a suppression of CMC. The response of irradiated mice treated with tumor allografts was restored with small numbers of spleen or lymph node cells from syngeneic or semi-allogeneic F/sub 1/ hybrid donors. With the use of the appropriate cytotoxic alloantisera, it was demonstrated that the majority of the effector cells generated in the spleens of mice restored with semiallogeneic cells were of host origin. These results demonstrate that the precursors of the cytotoxic lymphocytes are radioresistant and indicate that for their stimulation some radiosensitive T cells are necessary to amplify their reaction to nonlymphoid allografts. Allogeneic lymphoid cells, on the other hand, supply a stimulus which does not require the intervention of such amplifier cells. In this case, irradiation induces a stronger CMC response probably by inactivating radiosensitive cells with suppressor activity.

• Research Organization: Univ. of Manitoba, Winnipeg
• OSTI ID: 7190226
• Journal Information: Cell. Immunol.; (United States), Vol. 18:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
IMMUNE REACTIONS
BIOLOGICAL RADIATION EFFECTS
LYMPHOCYTES
RADIOSENSITIVITY
SYNERGISM
SPLEEN CELLS
THYMOCYTES
GAMMA RADIATION
IMMUNE SERUMS
MICE
TOXICITY
TRANSPLANTS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
LEUKOCYTES
MAMMALS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals