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Loss of stem cell function in the bone marrow of mice undergoing a graft-vs-host reaction

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5317849
;
The injection of parental T cells into healthy Fl hybrid mice can result in severe immunologic abnormalities including immune deficiency in the recipients. To test whether stem cell function could also be affected in Fl mice undergoing a parental-induced graft-vs-host (GVH) reaction, T cell-depleted bone marrow from parentally-injected Fl mice was tested for stem cell proliferative and T cell differentiation capacity by transplantation of the bone marrow from GVH mice into lethally irradiated syngeneic Fl test recipients. Stem cell proliferative capacity was tested in the repopulating spleens of the test mice by an in vivo pulse with /sup 125/IUDR. A reduction was observed in the proliferative capacity of marrow from GVH mice. From 6-to-10 weeks after grafting the test recipients, their spleen cells were tested in vitro for mitogen stimulation and for the generation of cytotoxic T lymphocytes (CTL) responses to hapten-self and alloantigens. No mitogen nor CTL responses were detected in the spleens of test mice inoculated with marrow from GVH mice. These findings indicate that the GVH reaction can affect early stages in the development of the lympho-hematopoietic system.
Research Organization:
NIH, Bethesda, MD
OSTI ID:
5317849
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
LETHAL IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
STEM CELLS
TISSUES
TRACER TECHNIQUES
TRANSPLANTS
VERTEBRATES