Loss of stem cell function in the bone marrow of mice undergoing a graft-vs-host reaction
The injection of parental T cells into healthy Fl hybrid mice can result in severe immunologic abnormalities including immune deficiency in the recipients. To test whether stem cell function could also be affected in Fl mice undergoing a parental-induced graft-vs-host (GVH) reaction, T cell-depleted bone marrow from parentally-injected Fl mice was tested for stem cell proliferative and T cell differentiation capacity by transplantation of the bone marrow from GVH mice into lethally irradiated syngeneic Fl test recipients. Stem cell proliferative capacity was tested in the repopulating spleens of the test mice by an in vivo pulse with /sup 125/IUDR. A reduction was observed in the proliferative capacity of marrow from GVH mice. From 6-to-10 weeks after grafting the test recipients, their spleen cells were tested in vitro for mitogen stimulation and for the generation of cytotoxic T lymphocytes (CTL) responses to hapten-self and alloantigens. No mitogen nor CTL responses were detected in the spleens of test mice inoculated with marrow from GVH mice. These findings indicate that the GVH reaction can affect early stages in the development of the lympho-hematopoietic system.
- Research Organization:
- NIH, Bethesda, MD
- OSTI ID:
- 5317849
- Report Number(s):
- CONF-8604222-; TRN: 86-028553
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
The graft-versus-host reaction and immune function. II. Recruitment of pre-T-cells in vivo by graft-versus-host-induced dysplastic thymuses following irradiation and bone marrow treatment
Studies of allogeneic bone marrow and spleen cell transplantation in a murine model using ultraviolet-B light
Related Subjects
BONE MARROW
TRANSPLANTS
HEMATOPOIETIC SYSTEM
GRAFT-HOST REACTION
LYMPHOCYTES
CELL PROLIFERATION
STEM CELLS
IODINE 125
LETHAL IRRADIATION
MICE
MITOGENS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals