Alpha-irradiation-induced G2 delay: a period of cell recovery

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575776· OSTI ID:5654759

Lucke-Huhle, C

Exponentially growing Chinese hamster V79 cells were delayed in G2 very efficiently by 3.4-MeV ..cap alpha.. particles. In comparison with the effect caused by sparsely ionizing /sup 60/Co ..gamma.. rays, G2 delay after ..cap alpha.. irradiation was greater by a factor of 6.7 and 4.2 for doses <0.5 Gy and >0.5 Gy, respectively, if the slopes of the dose-effect curves are compared. While at low doses (0.03-0.5 Gy) G2 arrest was reversible within 10 hr, increasing doses (0.5-4.38 Gy) of ..cap alpha.. irradiation blocked increasing fractions of cells for more than 16 hr, as determined by flow cytometry, and only some of these were able to complete mitosis. Addition of caffeine, however, reduced G2 arrest considerably if given directly after irradiation and reversed G2 arrest if added 8 hr after 4.38 Gy of ..cap alpha.. particles, a time when most of the cells already had accumulated in G2, caffeine treatment during G2 decreased survival after ..cap alpha.. irradiation by factors of 1.3 and 1.7 for 1 and 2 mM caffeine, respectively.

• Research Organization: Kernforschungszentrum Karlsruhe, Federal Republic of Germany
• OSTI ID: 5654759
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 89:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA BEAMS
ALPHA DECAY RADIOISOTOPES
ALPHA PARTICLES
AMERICIUM 241
AMERICIUM ISOTOPES
ANALEPTICS
ANIMAL CELLS
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BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
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BIOLOGICAL RECOVERY
CAFFEINE
CELL DIVISION
CENTRAL NERVOUS SYSTEM AGENTS
CHARGED PARTICLES
COBALT 60
COBALT ISOTOPES
COMPARATIVE EVALUATIONS
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DOSES
DRUGS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HAMSTERS
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HELIUM 4 BEAMS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ION BEAMS
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ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
MITOSIS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
RODENTS
VERTEBRATES
XANTHINES
YEARS LIVING RADIOISOTOPES