Production and response to an autologous growth factor isolated from human leukemia cells

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90232-2· OSTI ID:5623371

Heil, M F; Chiao, J W ^[1]; Leung, K ^[2]

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1. New York Medical College, Valhalla (United States)
2. Ohio State Univ., Columbus (United States)

Human myeloid leukemia HL-60 cells have been shown to release into culture medium an activity that promotes the proliferation of these same HL-60 cells. The presence of additional culture supernatant containing growth-promoter activity accelerates the HL-60 growth rate as determined by cell counts and ({sup 3}H)thymidine uptake. Isolation of this growth promoter has been performed with the serum-free supernatant of HL-60 cells using salt precipitation, DEAE-Sepharose ion exchange chromatography, and gel electrophoresis. The promoter activity was recovered from SDS-gel electrophoresis within the 60- to 85-kDa mol wt range where a single band of an apparent mol wt of 72,000 was demonstrated. The ability of HL-60 cells to respond to the growth promoter was significantly lost 4 hours after exposure to differentiation-inducing activity, while production of the growth promoter was diminished only after 2-3 days following induction of differentiation. These results suggest that the growth-promoting mechanism is associated with the undifferentiated leukemic state. In addition, the role of an autocrine growth mechanism in maintaining the leukemic cells in an undifferentiated state is discussed.

• OSTI ID: 5623371
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 180:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOSYNTHESIS
CELL DIFFERENTIATION
CELL PROLIFERATION
CHROMATOGRAPHY
DISEASES
ELECTROPHORESIS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IMMUNE SYSTEM DISEASES
ION EXCHANGE CHROMATOGRAPHY
LEUKEMIA
MAMMALS
MAN
MITOGENS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROTEINS
PYRIMIDINES
RIBOSIDES
SEPARATION PROCESSES
SYNTHESIS
THYMIDINE
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES