An oligomer complementary to c-myb-encoded mRNA inhibits proliferation of human myeloid leukemia cell lines

Journal Article · Mon May 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.9.3379· OSTI ID:5442859

Anfossi, G; Gewirtz, A M; Calabretta, B ^[1]

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1. Temple Univ. School of Medicine, Philadelphia, PA (USA)

To study the role of the protooncogene c-myb in regulating myeloid leukemia cell proliferation and differentiation, the authors exposed cells of the human leukemia lines HL-60, ML-3, KG-1, and KG-1a to an oligodeoxynucleotide complementary to an 18-base-pair (bp) sequence of c-myb-encoded mRNA. This treatment resulted in a significant decrease in cell proliferation in all of the lines, which was most marked in HL-60 cells. After 5 days in culture, in several separate experiments with different oligomer preparations, 75% growth inhibition was observed in c-myb antisense treated cells in comparison to untreated HL-60 cells. These studies indicate that the nuclear protein encoded by the c-myb protooncogene is required for maintenance of proliferation in certain leukemia cell lines. In addition, the stringent requirements for c-myb protein as compared to c-myc protein suggest that, at least in HL-60 cells, c-myc amplification or N-ras activation may not be sufficient to maintain the leukemic growth in the absence of c-myb protein. These findings support the hypothesis that development and maintenance of a malignant phenotype requires a multiplicity of interrelated genetic events.

• OSTI ID: 5442859
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:9; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CARCINOGENESIS
CELL DIFFERENTIATION
CELL PROLIFERATION
DISEASES
GENES
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
INHIBITION
LEUKEMIA
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ONCOGENES
ORGANIC COMPOUNDS
PATHOGENESIS
TUMOR CELLS