Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.4.1028· OSTI ID:6879610

Wickstrom, E L; Bacon, T A; Gonzalez, A; Freeman, D L; Lyman, G H; Wickstrom, E

The human promyelocytic leukemia cell line HL-60 overexpresses the c-myc protooncogene. A calculated secondary structure for c-myc mRNA placed the initiation codon in a bulge of a weakly based-paired region. Treatment of HL-60 cells with 5' d(AACGTTGAGGGGCAT) 3', complementary to the initiation codon and the next four codons of c-myc mRNA, inhibited c-myc protein expression in a dose-dependent manner. However, treatment of HL-60 cells with 5' d(TTGGGATAACACTTA) 3', complementary to nucleotides 17-31 of vesicular stomatits virus matrix protein mRNA, displayed no such effects. These results agree with analogous studies of normal human T lymphocytes, except that only one-third as much oligomer was needed for a comparable effect. Proliferation of HL-60 cells in culture was inhibited in a sequence-specific, dose-dependent manner by the c-myc-complementary oligomer, but neither the oligomer complementary to vesicular stomatitis virus matrix protein mRNA nor 5' d(CATTTCTTGCTCTCC) 3', complementary to nucleotides 5399-5413 of human immunodeficiency virus tat gene mRNA, inhibited proliferation. It thus appears that antisense oligodeoxynucleotides added to myc-transformed cells via culture medium are capable of eliciting sequence-specific, dose-dependent inhibition of c-myc protein expression and cell proliferation.

• Research Organization: Univ. of South Florida, Tampa (USA)
• OSTI ID: 6879610
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:4; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL PROLIFERATION
CYSTEINE
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
FLUORESCENCE
GENES
HEMIC DISEASES
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
INHIBITION
ISOTOPES
LEUKEMIA
LIGHT NUCLEI
LUMINESCENCE
MESSENGER-RNA
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
RNA
SULFUR 35
SULFUR ISOTOPES
THIOLS
TUMOR CELLS
UPTAKE