Interleukin 1 as an autocrine growth factor for acute myeloid leukemia cells

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.7.2369· OSTI ID:5188783

Cozzolino, F; Rubartelli, A; Aldinucci, D; Sitia, R; Torcia, M; Shaw, A; Di Guglielmo, R ^[1]

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1. Universita di Firenze (Italy)

Production of interleukin 1 (IL-1) by leukemic cells was studied in 13 cases of acute myeloid leukemia. Intracytoplasmic immunofluorescence studies showed that the cells invariably contained the cytokine. Endogenous labeling studies demonstrated that acute myeloid leukemia cells produced either only the 33-kDa propeptide or both the propeptide and the 17-kDa mature form of IL-1{beta}. The 33-kDa propeptide IL-1{alpha} was always produced but was less frequently released. Involvement of IL-1 in leukemic cell growth was investigated using two antibodies specific for IL-1 subtypes, which inhibited spontaneous cell proliferation in the six cases studied. After acid treatment of the cells, a surface receptor for IL-1 could be demonstrated, which mediated {sup 125}I-labeled IL-1-specific uptake by leukemic cells. Furthermore, recombinant IL-1{alpha} or IL-1{beta} induced significant cell proliferation in 10 12 cases. The above findings were uncorrelated with the cytologic type (French-American-British classification) of leukemia. The studies suggest that IL-1 may act as an autocrine growth factor in most cases of acute myeloid leukemia.

• OSTI ID: 5188783
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:7; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
CELL PROLIFERATION
CHEMICAL REACTIONS
CHEMISTRY
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
GROWTH FACTORS
HALOGENATION
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKEMIA
LYMPHOKINES
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PEPTIDES
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
TRACER TECHNIQUES
TUMOR CELLS