Induction of terminal differentiation in human promyelocytic leukemia cells by tumor-promoting agents. [Phorbol-12-myristate-13-acetate]

Journal Article · Thu Mar 01 04:00:00 EST 1979 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.76.3.1293· OSTI ID:6472016

Huberman, E; Callaham, M F

Human promyeloytic leukemia cells (HL-60) were induced to differentiation into mature cells by the tumor-promoting agent phorbol-12-myristate-13-acetate and other related phorbol diesters. Differentiation was determined by an increase in the percent of myelocytes, metamyelocytes, and other mature myeloid cells as well as by an increase in the percent of phagocytizing cells. Induction of differentiation could be determined after 2 days of treatment with phorbol-12-myristate-13-acetate at a dose as low as 6x10/sup -11/ M. A correlation was found between reported tumor-promoting activity of a series of phorbol esters and their ability to induce myeloid differentiation and to inhibit cell growth. It is suggested that tumor-promoting agents like chemicals that induce terminal differentiation in these cells, at extremely low concentrations, may be used as a tool in the study of the control of cell growth, cell differentiation, and malignancy in human leukemic cells.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 6472016
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 76:3; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL EFFECTS
CELL DIFFERENTIATION
DATA
DATA FORMS
DISEASES
ESTERS
EXPERIMENTAL DATA
GRAPHS
HEMIC DISEASES
INFORMATION
ISOLATED VALUES
LEUKEMIA
NEOPLASMS
NUMERICAL DATA
ORGANIC COMPOUNDS
TUMOR CELLS