Control of cell differentiation in cultured human cells by tumor promoting agents

Journal Article · Sun Apr 01 04:00:00 EST 1984 · Transplant. Proc.; (United States)

OSTI ID:5963830

Huberman, E

Unlike tumor initiators, the chemicals that promote tumor formation are devoid of mutagenic activity. These chemicals may exert their promotional effect by causing the expression of the mutated tumor genes in a process similar to gene expression during cell differentiation. Indeed, phorbol diesters and teleocidin have been shown to act as inducers of cell differentiation in a number of cell types. In cell differentiation studies with tumor promoters, the authors used 3 different human cell types, HO melanoma cells and the promyelocytic HL-60 and T lymphoid CEM leukemia cells. In the HO melanoma cells, the prototype phorbol diester phorbol-12-myristate-13-acetate (PMA), at doses as low as 10/sup -10/ to 10/sup -9/ M, induces a cell differentiation characterized by an inhibition of cell growth, increased synthesis of melanin, and induction of dendritelike structures. In this system, the authors demonstrated a relationship between the tumor-promoting activity of a series of phorbol diesters in vivo and the degree to which these agents induce differentiation in vitro. A similar relationship was demonstrated using the HL-60 cells.

• Research Organization: Argonne National Lab., IL
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5963830
• Journal Information: Transplant. Proc.; (United States), Journal Name: Transplant. Proc.; (United States) Vol. 16:2; ISSN TRPPA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
CARCINOGENESIS
CARCINOGENS
CELL CULTURES
CELL DIFFERENTIATION
ESTERS
IN VITRO
IN VIVO
MAMMALS
MAN
ORGANIC COMPOUNDS
PATHOGENESIS
PHORBOL ESTERS
PRIMATES
PROMOTERS
TOXICITY
TUMOR CELLS
TUMOR PROMOTERS
VERTEBRATES