Stimulation of differentiated functions in human melanoma cells by tumor-promoting agents and dimethyl sulfoxide

Journal Article · Sun Jul 01 04:00:00 EDT 1979 · Cancer Res.; (United States)

OSTI ID:5097638

Huberman, E; Heckman, C; Langenbach, R

Treatment of cultured human HO melanoma cells with the mouse skin tumor promoter phorbol-12-myristate-13-acetate (PMA) at 5 x 10/sup -10/ to 5 x 10/sup -7/ M resulted in a dose-related inhibition of growth and a stimulation of differentiated functions. These included melanin synthesis and formation of dendrite-like structues. Higher doses of phorbol dibutyrate, a less potent tumor promoter, were required to produce an effect comparable to that of PMA for dendrite induction. Phorbol and two other phorbal esters, which lack tumor-promoting activity, were either inactive or elicited a poor response. In addition to morphological changes, treatment with PMA altered glucosamine incorporation into membrane gangliosides. After PMA treatment, glucosamine incorporation increased 8- to 10 fold in the G/sub m3/ ganglioside and decreased 2-fold in the G/sub m1/ ganglioside, as compared to phorbol or untreated control. Inhibition of cell growth and stimulation of melanin synthesis were also observed after treatment of the HO cells with dimethyl sulfoxide. Unlike the tumor-promoting agents, dimethyl sulfoxide did not induce the formation of dendrite-like structures in the cells. These findings indicate that HO melanoma cells can be stimulated into terminally differentiated cells after treatment with tumor-promoting agents such as phorbol diesters.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5097638
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
CARCINOGENS
CELL CULTURES
COMPARATIVE EVALUATIONS
CRYSTALS
DENDRITES
DISEASES
DMSO
DOSE-RESPONSE RELATIONSHIPS
ESTERS
GROWTH
HYDROXY COMPOUNDS
INHIBITION
MAMMALS
MAN
MELANIN
MELANOMAS
MORPHOLOGICAL CHANGES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC POLYMERS
ORGANIC SULFUR COMPOUNDS
PIGMENTS
POLYESTERS
POLYMERS
PRIMATES
SULFOXIDES
TOXICITY
VERTEBRATES