Separation and purification procedure for (/sup 14/C) di-2-ethylhexyl phthalate in fish

Journal Article · · J. Anal. Toxicol.; (United States)

OSTI ID:5622386

Karara, A H; Hayton, W L; Archer, B G

A procedure utilizing the reverse isotope dilution analysis is presented for the determination of di-2-ethylhexyl phthalate (DEHP) and total metabolites in tissues of fish and water samples. This procedure offers several advantages: it eliminates laborious and expensive use of column chromatography and identification by gas chromatography/mass spectrometry analysis; it does not require quantitative separation; it selectively removes fish lipids by partitioning across a hexane/acetonitrile system; it uses an internal standard, di-pentylphthalate, for the gas-liquid chromatographic analysis; and it overcomes interference from background DEHP.

• Research Organization: Washington State Univ., Pullman
• OSTI ID: 5622386
• Journal Information: J. Anal. Toxicol.; (United States), Journal Name: J. Anal. Toxicol.; (United States) Vol. 8:3; ISSN JATOD
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400301* -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
ANIMALS
AQUATIC ORGANISMS
CARBON 14 COMPOUNDS
DATA
ESTERS
EXPERIMENTAL DATA
FISHES
HYDROGEN COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
LIPIDS
MEASURING METHODS
METABOLITES
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHTHALIC ACID ESTERS
PURIFICATION
SAMPLE PREPARATION
SAMPLING
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
WATER