Biotransformation of di(2-ethylhexyl)phthalate by rainbow trout
- RCG/Hagler Bailly, Inc., Boulder, CO (United States)
- National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)
- Ohio State Univ., Columbus, OH (United States). College of Pharmacy
The biotransformation of di(2-ethylhexyl)phthalate (DEHP) was studied in rainbow trout (Oncorhynchus mykiss) following intravascular administration. Methyl-esterified metabolites were identified using rodent-derived standards and non-linear gradient elution HPLC; metabolites were confirmed by gas chromatography. Similarities between the biotransformation of DEHP by rainbow trout and mammalian species included (a) mono-ethylhexyl phthalate (MEHP) appeared to be the obligatory first step in DEHP metabolism; (b) the phthalate ring was not oxidized; (c) phthalic acid was a minor metabolite; and (d) several metabolites contained multiple oxidations of the 2-ethylhexyl moiety of MEHP. No metabolites unique to rainbow trout were identified. However, fewer oxidized metabolites were identified in rainbow trout than in mammalian species, possibly due to limited mitochondrial metabolism of MEHP in rainbow trout. The amount of biliary MEHP glucuronide after intravascular administration of DEHP was substantially less than reported in rainbow trout exposed to DEHP via the water. Results confirmed that DEHP metabolism in rainbow trout proceeds by initial rapid formation of MEHP, followed by excretion or extensive oxidation by microsomal P450.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 64489
- Journal Information:
- Environmental Toxicology and Chemistry, Vol. 14, Issue 5; Other Information: PBD: May 1995
- Country of Publication:
- United States
- Language:
- English
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