Harvesting the noncirculating pool of polymorphonuclear leukocytes in rats by hetastarch exchange transfusion (HET): yield and functional assessment

Journal Article · Sun Nov 01 04:00:00 EST 1987 · J. Leukocyte Biol.; (United States)

OSTI ID:5603837

Williams, Jr, J H; Moser, K M; Ulich, T; Cairo, M S

Isolation of polymorphonuclear leukocytes (PMN) provides an opportunity to study PMN activity in vitro and to label PMN for study of in vivo kinetics. However, simple phlebotomy (SP) of a small animal frequently yields too few PMN for in vitro handling, while PMN harvested from an induced-peritonitis may not accurately reflect PMN in a less stimulated state. We report a novel method of harvesting PMN from the circulation of rats, using hetastarch exchange transfusion (HET), which is both time and animal sparing. HET harvested 8-fold more PMN than SP. In vitro cell function was examined with assays of adherence, chemotaxis, bacterial killing, and superoxide generation. No significant (p less than 0.05) difference was found between PMN obtained by HET and pooled-PMN obtained by SP. In vivo function was examined following labeling with indium 111-oxine. The kinetics pattern described suggested normal migratory activity when compared to previous reports. The data demonstrate that rats possess a relatively large, noncirculating pool of PMN which is readily accessible by HET.

• Research Organization: Univ. of California, Irvine
• OSTI ID: 5603837
• Journal Information: J. Leukocyte Biol.; (United States), Journal Name: J. Leukocyte Biol.; (United States) Vol. 42:5; ISSN JLBIE
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DISPERSIONS
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MIXTURES
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXINE
POLYSACCHARIDES
PYRIDINES
QUINOLINES
RADIOISOTOPES
RATS
REAGENTS
RODENTS
SACCHARIDES
SOLUTIONS
STARCH
THERAPY
TRACER TECHNIQUES
TRANSFUSIONS
VERTEBRATES