Function of irradiated polymorphonuclear leukocytes obtained by buffy-coat centrifugation
Several studies suggest that transfusion of polymorphonuclear leukocytes (PMNs) may be beneficial in the treatment of septic neonatal patients. Because of expense, donor availability, and the technical effort involved in obtaining PMNs by intermittent or continuous flow leukapheresis, buffy coat centrifugation of whole blood has been suggested as an alternative source. An in vitro study was performed to determine whether PMNs collected by this method have adequate oxidative and migratory function measured by chemiluminescence (CL) and chemotaxis under agarose (CT), respectively. Whole blood samples from six adult volunteers were drawn into citrate-phosphate-dextrose-adenine-one and stored at 4 degrees C for 0 to 48 hours. One-half of each sample was irradiated with 1500 rads. PMNs isolated from the buffy coat of these samples had greater than 80 percent normal CT and CL following 0 to 28 hours of storage in whole blood. Irradiation caused no depression in function. Units of whole blood yielded 1.11 +/- 0.40 X 10(9) PMNs per unit. This study indicates that transfusion of radiated PMNs obtained from stored whole blood that is less than 28 hours old is reasonable to use in studies involving PMN transfusions.
- Research Organization:
- Bowman Gray School of Medicine, Winston-Salem, NC
- OSTI ID:
- 6348427
- Journal Information:
- Transfusion (Philadelphia); (United States), Vol. 24:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LEUKOCYTES
BIOLOGICAL RADIATION EFFECTS
CENTRIFUGATION
CHEMILUMINESCENCE
GRAFT-HOST REACTION
IONIZING RADIATIONS
MAN
TRANSFUSIONS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
LUMINESCENCE
MAMMALS
MATERIALS
PRIMATES
RADIATION EFFECTS
RADIATIONS
SEPARATION PROCESSES
THERAPY
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)