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Indium-111-labeled human polymorphonuclear leukocytes: viability, random migration, chemotaxis, bactericidal capacity, and ultrastructure

Journal Article · · J. Nucl. Med.; (United States)
OSTI ID:5702844
; ; ; ; ;
Human polymorphonuclear leukocytes (PMNs) were labeled with indium-III (In-III) oxine in ethanol, and the effects of the labeling procedure, radioactivity, and concentrations of oxine and ethanol on PMN function and structure were studied in vitro. The standard labeling procedure did not alter the viability, random migration, chemotaxis, bactericidal capacity, or the ultrastructure of PMNs. Exposure to higher doses of radioactivity, or to higher concentrations of ethanol, had no appreciable effects on random migration and chemotaxis of PMNs. A dose-dependent reduction in their random migration and chemotaxis was observed when higher concentrations of oxine were used. These results indicate that In-III-labeled PMNs are structurally intact and have normal in vitro locomotion and bactericidal activity. Indium-III-labeled PMNs should be suitable for studying the kinetics and distribution of these cells in health and disease.
Research Organization:
Yale Univ. School of Medicine, New Haven, CT
OSTI ID:
5702844
Journal Information:
J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 20:7; ISSN JNMEA
Country of Publication:
United States
Language:
English

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Related Subjects

550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
560122* -- Radiation Effects on Cells-- Internal Source-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
ETHANOL
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INDIUM
LEUKOCYTES
METALS
MORPHOLOGICAL CHANGES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXINE
PHAGOCYTOSIS
PYRIDINES
QUINOLINES
ULTRASTRUCTURAL CHANGES
VIABILITY