Potential use of indium-111-labeled polymorphonuclear leukocytes for the detection of lung microvascular injury

Journal Article · Sun May 01 04:00:00 EDT 1988 · J. Nucl. Med.; (United States)

OSTI ID:7140395

Das, D K; Steinberg, H; Bandyopadhyay, D; Hoory, S

The early stages of microvascular injury are often difficult to detect due to the lack of a suitable marker to assess such an injury. We utilized the well known phenomenon of polymorphonuclear leukocyte (PMN) migration to the microvascular bed as a result of acute inflammatory reactions originating from the damaged cells. A radiotracer technique was developed, employing indium-111-labeled PMN for the detection of microvascular injury induced by hyperoxia. New Zealand white rabbits exposed to either 100% oxygen or air for various intervals of time were injected with indium-111-tropolone or oxine-labeled PMNs. Influx of radioactive PMN into the lung was detected in 72 hr/oxygen-exposed animals using gamma scintigraphic technique. Analysis of dry/wet ratios and histological examinations of the lung biopsies indicated noncardiogenic edema formation at this stage. Mortality was 50% beyond 96 hr/oxygen exposure. Our study thus provided a means to detect early microvascular injury during 72 hr/oxygen-exposure, which was not detectable by any other noninvasive techniques. The use of indium-111-labeled PMN thus appears to be a potentially important tool for the clinical assessment of lung microvascular injury.

• Research Organization: Univ. of Connecticut School of Medicine, Farmington (USA)
• OSTI ID: 7140395
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 29:5; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANOXIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
INDIUM 111
INDIUM ISOTOPES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LUNGS
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NEUTROPHILS
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOMETALLIC COMPOUNDS
ORGANS
OXYGEN
PATHOLOGICAL CHANGES
RABBITS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RESPIRATORY SYSTEM
SCINTISCANNING
SYMPTOMS
TRACER TECHNIQUES
USES
VASCULAR DISEASES
VERTEBRATES