Cholesterol biosynthesis in polychlorinated biphenyl-treated rats

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(82)90052-4· OSTI ID:5575585

Kling, D; Gamble, W

After administration of polychlorinated biphenly (PCB) at 0.055 (w/w) of the diet to Wistar rats for 30 days, followed by intraperitioneal injection of tritiated water, (/sup 14/C)mevalonate, and (/sup 14/C)acetate, there was a decrease in cholesterol biosynthesis in rat liver. No significant change in cholesterol formation was observed when PCB was administered at 0.01% (w/w) of the diet. In vitro inhibition of cholesterol synthesis by rat liver microsomes was observed with PCB. Squalene 2,3-oxidocyclase activity of rat liver microsomes was not significantly altered. Desmosterol delta/sup 24/ reductase activity was inhibited only at relatively high concentrations of PCB. There was increased incorporation of radioactivity into squalene and lanosterol, in vitro, in the presence of PCB. The primary inhibition of cholesterol biosynthesis appears to be at the demethylation and rearrangement reactions between lanosterol and cholesterol in the biosynthetic pathway.

• Research Organization: Oregon State Univ., Corvallis
• OSTI ID: 5575585
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 27:1; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CELL CONSTITUENTS
CHLORINATED AROMATIC HYDROCARBONS
CHOLESTEROL
DIET
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HALOGENATED AROMATIC HYDROCARBONS
HYDROXY COMPOUNDS
IN VITRO
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
MICROSOMES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANOIDS
ORGANS
RATS
RODENTS
STEROIDS
STEROLS
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES