The effects of phosphate on the biosynthesis of cholesterol in rat liver homogenates
The biosyntheses of cholesterol from acetate and mevalonate were determined in rat liver homogenates that were prepared and incubated in buffers containing varying concentrations of phosphate. Relatively little acetate or mevalonate was incorporated into cholesterol in the absence of added phosphate. When phosphate was added, there was an increase in incorporation of both substrates. The addition of phosphate resulted in an increase in the incorporation of mevalonate to a maximum, whereas phosphate appeared to increase the incorporation of acetate at low phosphate levels and decrease the incorporation at higher phosphate levels. The results appear to be consistent with the possibility that, at low phosphate levels, the biosynthesis of cholesterol is limited by some phosphate-requiring reaction(s) in the pathway after mevalonate, and at higher phosphate levels, the biosynthesis is limited by the 3-hydroxy-3-methylglutaryl coenzyme A reductase-catalyzed step.
- Research Organization:
- Department of Biochemistry, Eastern Virginia Medical School, Norfolk
- OSTI ID:
- 5987191
- Journal Information:
- Lipids; (United States), Journal Name: Lipids; (United States) Vol. 17:10; ISSN LPDSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ACID PHOSPHATES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBON ISOTOPES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHOLESTEROL
DIGESTIVE SYSTEM
GLANDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIVER
MAMMALS
METABOLISM
MEVALONIC ACID
NORTH AMERICA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PHYSIOLOGY
RATS
REACTION KINETICS
RODENTS
STEROIDS
STEROLS
SYNTHESIS
TRACER TECHNIQUES
USA
VERTEBRATES
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ACID PHOSPHATES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBON ISOTOPES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHOLESTEROL
DIGESTIVE SYSTEM
GLANDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIVER
MAMMALS
METABOLISM
MEVALONIC ACID
NORTH AMERICA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PHYSIOLOGY
RATS
REACTION KINETICS
RODENTS
STEROIDS
STEROLS
SYNTHESIS
TRACER TECHNIQUES
USA
VERTEBRATES