Cell-survival characteristics of intestinal stem cells and crypts of. gamma. -irradiated mice

Journal Article · Mon Oct 01 04:00:00 EDT 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575118· OSTI ID:5570475

Yau, H C; Cairnie, A B

Ninety-one female Swiss Webster mice which survived to 4 days after whole-body irradiation (/sup 137/Cs-..gamma.. rays, 102 rad/min) were sacrificed for assay of the number of surviving crypts. (/sup 3/H)Thymidine was injected 45 min prior to sacrifice and crypt survival determined from /sup 3/H counts per crypt, /sup 3/H counts per milligram of dry intestine, and the dry weight of the whole intestine after single doses of 960 to 1924 rad or split doses (4-hr interval) of 1155 + 385 to 962 rad. For the two highest doses in each series, for which crypts were very sparse, the /sup 3/H-counting procedure was replaced by a visual count in a specimen large enough to be weighed. The results were analyzed using, for comparison, various mathematical models. The best fit was obtained with nonlinear regression using a multitarget-with-two-components model. The parameters of this curve are D/sub 0/, 109 rad; n (cell extrapolation number), 55; and A (stem cells per crypt), 31. The results are compared with those obtained by other workers.

• Research Organization: Queen's Univ., Kingston, Ontario
• OSTI ID: 5570475
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 80:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL KILLING
CESIUM 137
CESIUM ISOTOPES
COMPARATIVE EVALUATIONS
CRYPT CELLS
DATA
DATA FORMS
DIGESTIVE SYSTEM
DOSE RATES
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
EXTERNAL IRRADIATION
FRACTIONATED IRRADIATION
GAMMA RADIATION
GASTROINTESTINAL TRACT
INFORMATION
INTESTINES
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
ISOTOPES
MAMMALS
MATHEMATICAL MODELS
MICE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SMALL INTESTINE
SOMATIC CELLS
STEM CELLS
SURVIVAL CURVES
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES