Interaction of radiation and cyclophosphamide in intestinal crypt cells
LAF/sub 1/ mice were used to study the interaction of radiation and cyclophosphamide using the intestinal crypt survival technique. The LD/sub 50/60/ for cyclophosphamide was 340 mg/kg and the MTD (LD/sub 01/) was 250 mg/kg. The MTD dose caused only 35% kill of intestinal crypt cells when given 3 hr after 1100 rad. The results were the same when drug was given 2 hr before irradiation. MTD doses given from -48 to +48 hr relative to a radiation dose of 1100 rad caused survival fluctuations from 10 to 50 crypt cells with minima at -18 and +12 hr and maxima at -36 and +24 hr, suggesting a periodicity of about 30 hr. Radiation survival curves determined 2 hr after administration of the MTD have the same slope as controls and the extrapolated curve yields a D/sub q/ of 400 rad. A 3 hr interval, 2-dose experiment yielded a survival ratio of 6 for control and 15 for cyclophosphamide treated animals. Cyclophosphamide appears to have minimal activity in intestinal crypt cells and there does not appear to be any interaction between the drug and radiation lesions.
- Research Organization:
- Univ. of California, San Francisco
- DOE Contract Number:
- EY-76-C-03-1012
- OSTI ID:
- 5237286
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 5:9; ISSN IOBPD
- Country of Publication:
- United States
- Language:
- English
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560121* -- Radiation Effects on Cells-- External Source-- (-1987)
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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
ANTIMITOTIC DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CESIUM 137
CESIUM ISOTOPES
CRYPT CELLS
DATA
DATA FORMS
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOXAN
EXPERIMENTAL DATA
EXTERNAL IRRADIATION
GAMMA RADIATION
GASTROINTESTINAL TRACT
INFORMATION
INTESTINES
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
ISOTOPES
MAMMALS
MICE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RODENTS
SMALL INTESTINE
SOMATIC CELLS
SURVIVAL CURVES
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES