Interaction of radiation and bleomycin in intestinal crypt cells
Using the intestinal crypt cell system, the effects of bleomycin (BLM) with radiation have been determined. The LD/sub 50/60/ in LAF/sub 1/ mice used was 167 units/kg and the MTD (LD/sub 01/) was 100 units/kg. Survival of crypt cells was reduced to 40% of radiation alone when drug was given 3 hr after a dose of 1100 rad, but to less than 1% when the drug was given 2 hr before. When maximum tolerated doses (MTD) were given from -48 to +48 hr relative to a dose of 843 rad, the combination was much more effective when drug was given from -12 to -2 hr before irradiation, survival dropping from 100 to 5 cells per circumference. This increased cell kill could be due to synchrony, interference with repair of BLM damage, or other factors. A radiation survival curve determined 2 hr after drug administration had a slope similar to controls, but was shifted toward lower doses. The extrapolated D/sub q/ was 0 rad. A 3 hr split dose experiment yielded survival ratios of 6 for controls and 2 for mice given the MTD 2 hr before irradiation. There appears to be a marked interaction of bleomycin and radiation, with possible inhibition of repair of the radiation damage by bleomycin and possible inhibition of repair of the bleomycin damage by radiation.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 5236359
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 5:9; ISSN IOBPD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLEOMYCIN
BODY
CESIUM 137
CESIUM ISOTOPES
CRYPT CELLS
DATA
DATA FORMS
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DOSES
DRUGS
EXPERIMENTAL DATA
EXTERNAL IRRADIATION
GASTROINTESTINAL TRACT
INFORMATION
INHIBITION
INTESTINES
IRRADIATION
ISOLATED VALUES
ISOTOPES
MAMMALS
MICE
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANS
RADIATION DOSES
RADIATION EFFECTS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
RODENTS
SMALL INTESTINE
SOMATIC CELLS
SURVIVAL CURVES
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES