Enhancement of the Hepatotoxicity of chloroform in B6C3F1 mice by corn oil: implications for chloroform carcinogenesis
A recent study of the ability of chloroform in drinking water to produce cancer reported that male Osborne-Mendel rats developed renal tumors, but that female B6C3F1 mice failed to develop hepatocellular carcinomas. The results obtained in the male Osborne-Mendel rats were comparable to those observed in an earlier study sponsored by the National Cancer Institute (NCI). On the other hand, the lack of an increased incidence of hepatocellular carcinomas in female B6C3F1 mice was in sharp contrast to previously reported results. The doses of chloroform used were comparable to that which produced an 85% incidence in the NCI study. The authors have investigated the extent to which the vehicle might be responsible for the different results in these two studies by examining the differential effects of chloroform when it was administered by gavage using corn oil versus a 2% Emulphor suspension as the vehicle. Male and female B6C3F1 mice were administered chloroform at 60, 130, and 270 mg/kg per day for 90 days. At sacrifice, body and organ weights were measured, and blood was recovered to perform the following serum chemistry measurements (in order of priority): glutamate oxalacetate transaminase (SGOT), lactate dehydrogenase (LDH), blood urea nitrogen (BUN), and triglyceride (TG) levels. The liver was sectioned for histopathological examination. These data indicate that administration of chloroform by corn oil gavage results in more marked hepatotoxic effects than observed when it is provided in an aqueous suspension. A major difference between two recent carcinogenesis bioassays of chloroform in this same mouse strain was the vehicle used.
- Research Organization:
- Washington State Univ., Pullman, WA (United States)
- OSTI ID:
- 5554984
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 69
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHLOROFORM
BIOLOGICAL EFFECTS
CORN OIL
LIVER
PATHOLOGICAL CHANGES
AMINOTRANSFERASES
BLOOD CHEMISTRY
CARCINOGENESIS
CARCINOMAS
ENZYME ACTIVITY
HISTOLOGY
LACTATE DEHYDROGENASE
MICE
TRIGLYCERIDES
UREA
AMIDES
ANIMALS
BODY
CARBONIC ACID DERIVATIVES
CHLORINATED ALIPHATIC HYDROCARBONS
DIGESTIVE SYSTEM
DISEASES
ENZYMES
ESTERS
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
HEMIACETAL DEHYDROGENASES
LIPIDS
MAMMALS
NEOPLASMS
NITROGEN TRANSFERASES
OILS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OTHER ORGANIC COMPOUNDS
OXIDOREDUCTASES
PATHOGENESIS
RODENTS
TRANSFERASES
VEGETABLE OILS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology