Heterogeneity in mouse seminal vesicle epithelial cells responding to androgen as evaluated by incorporation of (/sup 125/I)iododeoxyuridine

Journal Article · Mon Apr 01 04:00:00 EST 1985 · Endocrinology; (United States)

OSTI ID:5554050

Terada, N; Ogasawara, Y; Yamane, T; Matsumoto, K; Kitamura, Y

When the uptake of 5-(/sup 125/I)iodo-2'-deoxyuridine ((/sup 125/I)IdUrd) into the seminal vesicle of castrated mice was measured 3 days after starting injections of various doses of testosterone propionate (TP), logarithmic values of (/sup 125/I)IdUrd uptake were proportional to the logarithmic doses of TP in the range of 0.04-2 micrograms/g BW. The (/sup 125/I)IdUrd uptake values correlated well with the labeling and mitotic indices of epithelial cells. Since daily injections of 0.4 microgram TP/g BW did not increase significantly the weight or DNA content or protein content of the seminal vesicle, the (/sup 125/I)IdUrd uptake is a sensitive index of androgen action. Moreover, this suggests that low doses of androgen induce division of epithelial cells without resulting in the increase in cell number. The (/sup 125/I)IdUrd radioactivity in the seminal vesicle was measured 2-15 days after the injection of (/sup 125/I)IdUrd, since the value represented the fraction of surviving cells synthesizing DNA at the time of (/sup 125/I)IdUrd injection. When injections of 4 micrograms TP/g BW were continued, the incorporated radioactivity was retained. In contrast, continuous injections of 0.2 microgram TP/g BW did not maintain the radioactivity, of which incorporation was induced by the same dose of TP. Thus, the present result suggests the presence of heterogeneity in androgen-responsive epithelial cells of the seminal vesicle.

• Research Organization: Osaka Univ. Medical School, Japan
• OSTI ID: 5554050
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 116:4; ISSN ENDOA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BODY
CASTRATION
CELL DIVISION
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IODOURACILS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MALE GENITALS
MAMMALS
MEDICINE
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SURGERY
TESTOSTERONE
TRACER TECHNIQUES
UPTAKE
URACILS
VERTEBRATES