Radiosensitivity of mouse seminal vesicle cells which show proliferative response to androgen and estrogen

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576194· OSTI ID:5245076

Okamoto, S; Ogasawara, Y; Kitamura, Y; Matsumoto, K; Mori, K J

Injections of either androgen or estrogen have been shown to induce proliferation of epithelial cells in the seminal vesicle of castrated mice. Uptake of 5-(/sup 125/I)iodo-2'-deoxyuridine ((/sup 125/I)IdUrd) by the whole seminal vesicle was used as an index for cell proliferation. Although uptake of (/sup 125/I)IdUrd induced by androgen was about four times as great as that induced by estrogen, both values decreased with a similar pattern after irradiation. Uptake of (/sup 125/I)IdUrd showed a dose-dependent decrease up to 1000 rad; the values remained unchanged until 4000 rad. Uptake of (/sup 125/I)IdUrd by the radiosensitive cell population was calculated by substracting (I-125)IdUrd uptake attributable to the radioresistant cell population from total (I-125)IdUrd uptake. Androgen- and estrogen-responsive cells were equally sensitive to irradiation. Recovery of androgen-responsive cells from radiation-induced decrease was examined with or without androgen stimulation. Although recovery occurred without androgen, it was significantly enhanced by androgen stimulation following irradiation. Irradiation seems useful for investigation of kinetic characteristics of epithelial stem cells in the seminal vesicle of mice.

• Research Organization: Osaka Univ. Medical School, Osaka, Japan
• OSTI ID: 5245076
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 97:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550301 -- Cytology-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL PROLIFERATION
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
ESTROGENS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONIZING RADIATIONS
ISOTOPES
LABELLED COMPOUNDS
MALE GENITALS
MAMMALS
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
RODENTS
SOMATIC CELLS
STEM CELLS
STEROID HORMONES
STEROIDS
TISSUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES
X RADIATION