Relationship of changing delta 4-steroid 5 alpha-reductase activity to (125I)iododeoxyuridine uptake during regeneration of involuted rat prostates

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Biology of Reproduction; (USA)

DOI:https://doi.org/10.1095/biolreprod40.4.793· OSTI ID:5547626

Kitahara, S; Higashi, Y; Takeuchi, S; Oshima, H ^[1]

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1. Tokyo Medical and Dental Univ. (Japan)

To elucidate the phenotypic expression of proliferating prostatic cells, rats were castrated, and the regenerating process of involuted ventral prostates during testosterone propionate (TP) administration was investigated by examining morphology, (5-{sup 125}I)iododeoxyuridine ({sup 125}I-UdR) uptake, DNA content, weight, acid phosphatase, and delta 4-steroid 5 alpha-reductase (5 alpha-reductase) activities. Morphologically, TP treatment initially increased the number of epithelial cells lining glandular lobules and subsequently restored the shape of epithelial cells. {sup 125}I-UdR uptake peaked on Day 3 of TP treatment and stayed at higher levels than for uncastrated controls until Day 14 of treatment. Prostatic weight, protein content, acid phosphatase, and DNA content returned to uncastrated control levels by Day 14 of TP treatment. TP administration markedly stimulated prostatic 5 alpha-reductase activity, which peaked on the Day 5 of treatment and decreased to uncastrated control levels by Day 14 of treatment. It is concluded that TP administration to castrated rats initially induced active mitotic division of the remaining stem cells, followed by formation of differentiated functional epithelial cells. Prostatic 5 alpha-reductase was highly active at the initial phase of active mitotic cell division. The major portion of the increased enzyme activity can be regarded as a phenotypic expression of stem or transient cells of prostatic epithelium.

• OSTI ID: 5547626
• Journal Information: Biology of Reproduction; (USA), Journal Name: Biology of Reproduction; (USA) Vol. 40:4; ISSN BIREB; ISSN 0006-3363
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID PHOSPHATASE
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIMETABOLITES
AZINES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BODY
CELL PROLIFERATION
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
EPITHELIUM
ESTERASES
GLANDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IODODEOXYURIDINE
IODOURACILS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
MALE GENITALS
MAMMALS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHENOTYPE
PHOSPHATASES
PROSTATE
PYRIMIDINES
RADIOISOTOPES
RATS
REACTION KINETICS
RECOVERY
RIBOSIDES
RODENTS
SOMATIC CELLS
STEM CELLS
STEROID HORMONES
STEROIDS
TESTOSTERONE
TISSUES
TRACER TECHNIQUES
URACILS
VERTEBRATES
WEIGHT