Regulation of enzymes of serine and one-carbon metabolism by testosterone in rat prostate, liver, and kidney

Journal Article · Wed Oct 01 04:00:00 EDT 1975 · Endocrinology; (United States)

OSTI ID:7332104

Sanborn, T A; Kowle, R L; Sallach, H J

A significant decrease in the specific activity of 3 enzymes of serine and one-carbon metabolism (3-phosphoglycerate dehydrogenase, phosphoserine phosphatase, and serine hydroxymethyltransferase) was found in the rat prostate gland with castration. A single injection of testosterone propionate to rats 3 days after castration resulted in a significant increase in the 3 enzyme activities within 24 h. This increase in specific activity was maximal 72 h after injection of testosterone in the case of 3-phosphoglycerate dehydrogenase and serine hydroxymethyltransferase. When cycloheximide was administered in conjunction with testosterone, the increase in 3-phosphoglycerate dehydrogenase and serine hydroxymethyltransferase activity was significantly reduced compared to injection of testosterone alone. N/sup 6/,O/sup 2/'-dibutyryl adenosine-3',5'-cyclic monophosphate ((Bu)/sub 2/ cAMP) and theophylline injected at 8 h intervals to rats 3 days after castration failed to mimic the action of testosterone on these 3 enzymes 24 and 72 h after beginning injections. Castration had no effect on the specific activity of these enzymes in the kidney; however, 3-phosphoglycerate dehydrogenase was significantly diminished in the liver 6 days after castration. A single injection of testosterone to rats 3 days after castration restored the activity to sham-operated levels. Serine hydroxymethyltransferase and phosphoserine phosphatase activity in the liver were unaffected 6 days after castration. Thus testosterone exerts a regulatory role on serine and one-carbon metabolism in the prostate and liver which (Bu)/sub 2/ cAMP is unable to mimic.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 7332104
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 97:4; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANDROGENS
ANDROSTANES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HORMONES
HYDROXY ACIDS
HYDROXY COMPOUNDS
KETONES
KIDNEYS
LIVER
MALE GENITALS
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHYSIOLOGY
PROSTATE
RATS
RODENTS
SERINE
STEROID HORMONES
STEROIDS
TESTOSTERONE
VERTEBRATES