Inhibition of methanogenesis and carbon metabolism in Methanosarcina sp. by cyanide

Journal Article · Mon Apr 01 04:00:00 EST 1985 · J. Bacteriol.; (United States)

OSTI ID:5553884

Smith, M R; Lequerica, J L; Hart, M R

NaCN was tested for its inhibitory effects on growth of and metabolism by Methanosarcina barkeri 227. NaCN (10 ..mu..M) inhibited catabolism of acetate methyl groups to CH/sub 4/ and CO/sub 2/ but did not inhibit methanogenesis from methanol, CO/sub 2/, methylamine, or trimethylamine. NaCN also inhibited the assimilation of methanol or CO/sub 2/ (as the sole carbon source) into cell carbon and stimulated the assimilation of acetate. These results suggest that inhibition by NaCN was a result of its action as an inhibitor of in vivo CO dehydrogenase. The results also implicate CO dehydrogenase in the oxidation of acetate but not methanol groups to CO/sub 2/.

• Research Organization: Dept. of Agriculture, Berkeley, CA
• OSTI ID: 5553884
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 162:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ALCOHOLS
ALKALI METAL COMPOUNDS
ALKANES
AMINES
BACTERIA
BIOLOGICAL EFFECTS
CARBON
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CATABOLISM
CHALCOGENIDES
CYANIDES
ELEMENTS
ENZYME INHIBITORS
ENZYMES
GROWTH
HYDROCARBONS
HYDROLASES
HYDROXY COMPOUNDS
INHIBITION
METABOLISM
METHANE
METHANOGENIC BACTERIA
METHANOL
METHYLAMINE
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
SODIUM COMPOUNDS