Methanosarcina mutant unable to produce methane or assimilate carbon from acetate

Journal Article · Fri Nov 01 04:00:00 EST 1985 · J. Bacteriol.; (United States)

OSTI ID:5459826

Smith, M R; Lequerica, J L

Mutants of Methanosarcina barkeri 227 resistant to monofluoroacetate were isolated from monofluoroacetate-treated cultures. Mutant strain FA/sup r/9 was 100 times more resistant to monofluoroacetate than the wild-type strain and was deficient in carbon uptake and CH/sub 4/ and CO/sub 2/ production from methyl-labeled acetate. Methanol was assimilated at increased levels. Strain FA/sup r/9 was unable to shift from using methanol to using acetate for growth and exhibited increased sensitivity to growth inhibition by NaCN in methanol-containing complex medium. Unlike parent strain 227, acetate addition to methanol-containing media did not prevent NaCN inhibition. The specific activities of enzymes of exogenous acetate assimilation, CO dehydrogenase, and enzymes of the tricarboxylic acid cycle were similar for mutant and parent strain cell extracts. The authors conclude that strain FA/sup r/9 is either an acetate permeability mutant or is defective in an activation step required for the catabolism and anabolism of acetate.

• Research Organization: Dept. of Agriculture, Berkeley, CA
• OSTI ID: 5459826
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 164:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALKANES
BACTERIA
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL ACTIVATION
HYDROCARBONS
METABOLISM
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
MUTANTS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PERMEABILITY