Effects of hydrogen pressure during growth and effects of pregrowth with hydrogen on acetate degradation by Methanosarcina species. [Methanosarcina barkeri; Methanosarcina mazei]

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:7034395

Boone, D R; Menaia, J A.G.F.; Boone, J E; Mah, R A

Methanosarcina barkeri 227 and Methanosarcina mazei S-6 grew with acetate as the substrate; little effect of H/sub 2/ on the rate of aceticlastic growth was found in the presence of various H/sub 2/ pressures between 2 and 810 Pa. Physical (H/sub 2/ addition or flushing the headspace to remove H/sub 2/) and biological (H/sub 2/-producing or -utilizing bacteria in cocultures) methods were used for controlling H/sub 2/ pressure in Methanosarcina cultures growing on acetate. Added H/sub 2/ (ca. 100 Pa) was removed rapidly (a few hours) by M. barkeri and slowly (within a day) by M. mazei. When the H/sub 2/ produced by the aceticlastic methanogens was removed by coculturing with an H/sub 2/-using Desulfovibrio sp., the H/sub 2/ pressure was about 2.2 Pa. Under these conditions the stoichiometry of aceticlastic methanogenesis did not change. H/sub 2/-grown inocula of M. barkeri grew with acetate as the sole catabolic substrate if the inoculum culture was transferred during logarithmic growth to acetate-containing medium or if the transfer was accomplished with 1 or 2 days after exhaustion of H/sub 2/. H/sub 2/-grown cultures incubated for 4 or more days after exhaustion of H/sub 2/ were able to grow with H/sub 2/ but not with acetate as the sole catabolic substrate. Addition of small quantities of H/sub 2/ to acetate-containing medium permitted these cultures to initiate growth on acetate.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 7034395
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 53:1; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALKANES
BACTERIA
BIODEGRADATION
BIOSYNTHESIS
CARBOXYLIC ACID SALTS
CATABOLISM
CHEMICAL REACTIONS
DECOMPOSITION
DESULFOVIBRIO
ELEMENTS
GROWTH
HYDROCARBONS
HYDROGEN
METABOLISM
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
PRESSURE EFFECTS
STOICHIOMETRY
SULFATE-REDUCING BACTERIA
SYNTHESIS