Anaerobic degradation of lactate by syntrophic associations of methanosarcina barkeri and desulfovibrio species and effect of H2 on acetate degradation
When grown in the absence of added sulfate, cocultures of Desulfovibrio desulfuricans or Desulfovibrio vulgaris with Methanobrevibacter smithii (Methanobacterium ruminantium), which uses H2 and CO2 for methanogenesis, degraded lactate, with the production of acetate and CH4. When D. desulfuricans or D. vulgaris was grown in the absence of added sulfate in coculture with Methanosarcina barkeri (type strain), which uses both H2-CO2 and acetate for methanogenesis, lactate was stoichiometrically degraded to CH4 and presumably to CO2. During the first 12 days of incubation of the D. desulfuricans-M. barkeri coculture, lactate was completely degraded, with almost stoichiometric production of acetate and CH4. Later, acetate was degraded to CH4 and presumably to CO2. In experiments in which 20 mM acetate and 0 to 20 mM lactate were added to D. desulfuricans-M. barkeri cocultures, no detectable degradation of acetate occurred until the lactate was catabolized. The ultimate rate of acetate utilization for methanogenesis was greater for those cocultures receiving the highest levels of lactate. A small amount of H2 was detected in cocultures which contained D. desulfuricans and M. barkeri until after all lactate was degraded. The addition of H2, but not of lactate, to the growth medium inhibited acetate degradation by pure cultures of M. barkeri. Pure cultures of M. barkeri produced CH4 from acetate at a rate equivalent to that observed for cocultures containing M. barkeri. Inocula of M. barkeri grown with H2-CO2 as the methanogenic substrate produced CH4 from acetate at a rate equivalent to that observed for acetate-grown inocula when grown in a rumen fluid-vitamin-based medium but not when grown in a yeast extract-based medium. The results suggest that H2 produced by the Desulfovibrio species during growth with lactate inhibited acetate degradation by M. barkeri. (Refs. 26).
- Research Organization:
- Dept. of Dairy Science and Microbiology, Univ. of Illinois, Urbana, IL 61801
- OSTI ID:
- 5347978
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 41:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LACTATES
BIODEGRADATION
METHANE
PRODUCTION
ACETATES
ANAEROBIC DIGESTION
CARBON DIOXIDE
CELL CULTURES
CULTURE MEDIA
HYDROGEN
METHANOGENIC BACTERIA
ALKANES
BACTERIA
BIOCONVERSION
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL REACTIONS
DECOMPOSITION
DIGESTION
ELEMENTS
HYDROCARBONS
MANAGEMENT
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROCESSING
WASTE MANAGEMENT
WASTE PROCESSING
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
090122 - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)