Methanogenesis from choline by a coculture of Desulfovibrio sp. and Methanosarcina barkeri

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5627787

Fiebig, K; Gottschalk, G

A sulfate-reducing vibrio was isolated from a methanogenic enrichment with choline as the sole added organic substrate. This oganism was identified as a member of the genus Desulfovibrio and was designated Desulfovibrio strain G1. In a defined medium devoid of sulfate, a pure culture of Desulfovibrio strain G1 fermented choline to trimethylamine, acetate, and ethanol. In the presence of sulfate, more acetate and less ethanol were formed from choline than in the absence of sulfate. When grown in a medium containing sulfate, a coculture of Desulfovibrio strain G1 and Methanosarcina barkeri strain Fusaro degraded chloline almost completely to methane, ammonia, and hydrogen sulfide and presumably to carbon dioxide. Methanogenesis occurred in two distinct phases separated by a lag of about 6 days. During the first phase of methanogenesis choline was completely converted to trimethylamine, acetate, hydrogen sulfide, and traces of ethanol by the desulfovibrio.M. barkeri fermented trimethylamine to methane, ammonia, and presumably carbon dioxide via dimethyl- and methylamine as intermediates. Simultaneously, about 60% of the acetate expected was metabolized. In the second phase of methanogenesis, the residual acetate was almost completely catabolized.

• Research Organization: Institute for Mikrobiologie der Universitat Gottingen, Federal Republic of Germany
• OSTI ID: 5627787
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 45:1; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

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ALCOHOLS
ALKANES
AMINES
AMMONIUM COMPOUNDS
ANAEROBIC CONDITIONS
BACTERIA
BIOCONVERSION
BIODEGRADATION
BIOSYNTHESIS
CELL CULTURES
CHEMICAL REACTIONS
CHOLINE
DECOMPOSITION
DESULFOVIBRIO
DRUGS
FERMENTATION
HYDROCARBONS
HYDROXY COMPOUNDS
LIPOTROPIC FACTORS
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
QUATERNARY COMPOUNDS
SULFATES
SULFUR COMPOUNDS
SYNTHESIS