Bioconversion of gelatin to methane by a coculture of Clostridium collagenovorans and Methanosarcina barkeri

Journal Article · Wed Feb 01 04:00:00 EST 1989 · Applied and Environmental Microbiology; (USA)

OSTI ID:5161172

Jain, M K; Zeikus, J G ^[1]

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1. Michigan Biotechnology Institute, Lansing (USA)

A simple, stable, and transferable coculture of Clostridium collagenovorans and Methanosarcina barkeri that readily degraded gelatin into methane and carbon dioxide was developed. In monoculture, C. collagenovorans fermented all of the amino acids in gelatin except proline into acetate and carbon dioxide as the main products, with hydrogen, isovalerate, and isobutyrate detected in trace amounts (<1 mM). In coculture with M. barkeri, gelatin was transformed into methane and carbon dioxide, with varying levels of intermediary acetate formed as a function of incubation time. Various complex proteinaceous polymers could be readily transformed into methane and carbon dioxide at 30 to 40{degree}C by a stable coculture which did not require exogenous growth factor additions. In addition, the coculture was readily transferable and preserved in the viable state for long periods, and methanogenesis could be initiated rapidly without the need for exogenous pH control.

• OSTI ID: 5161172
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 55:2; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
ACETATES
ALKANES
ANAEROBIC DIGESTION
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CLOSTRIDIUM
COLLOIDS
DIGESTION
DISPERSIONS
GELATIN
HYDROCARBONS
MANAGEMENT
METABOLISM
METHANE
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROCESSING
PROTEINS
SYNTHESIS
WASTE MANAGEMENT
WASTE PROCESSING