Influence of corrinoid antagonists on methanogen metabolism
Iodopropane inhibited cell growth and methane production when Methanobacterium thermoautotrophicum, Methanobacterium formicicum, and Methanosarcina barkeri were cultured on H/sub 2/-CO/sub 2/. Iodopropane (40 ..mu..M) inhibited methanogenesis (30%) and growth (80%) when M. barkeri was cultured mixotrophically on H/sub 2/-CO/sub 2/-methanol. The addition of acetate to the medium prevented the observed iodopropane-dependent inhibition of growth. Cultivation of M. barkeri on H/sub 2/-CO/sub 2/-methanol in bright light also inhibited growth and methanogenesis to a greater extent in the absence than in the presence of acetate in the medium. Acetate was the only organic compound examined that prevented iodopropane-dependent inhibition of one-carbon metabolism in M. barkeri. The effect of iodopropane and acetate on the metabolic fates of methanol and carbon dioxide was determined with /sup 14/C tracers when M. barkeri was grown mixotrophically on H/sub 2/-CO/sub 2/-methanol. The corrinoid antagonists, light and iodopropane, appeared most specific for methanogen metabolic reactions involved in acetate synthesis from one-carbon compounds and acetate catabolism.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 6497162
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 146:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
BACTERIA
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
EVALUATION
GROWTH
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METHANATION
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
RADIATIONS
TRACER TECHNIQUES
VISIBLE RADIATION