Effect of angiotensin II, ATP, and ionophore A23187 on potassium efflux in adrenal glomerulosa cells

Journal Article · Sat Feb 01 04:00:00 EST 1986 · Am. J. Physiol.; (United States)

OSTI ID:5529205

Lobo, M V; Marusic, E T

Angiotensin II stimulus on perifused bovine adrenal glomerulosa cells elicited an increase in 86Rb efflux from cells previously equilibrated with the radioisotope. When 45Ca fluxes were measured under similar conditions, it was observed that Ca and Rb effluxes occurred within the first 30 s of the addition of the hormone and were independent of the presence of external Ca. The 86Rb efflux due to angiotensin II was inhibited by quinine and apamin. The hypothesis that the angiotensin II response is a consequence of an increase in the K permeability of the glomerulosa cell membrane triggered by an increase in cytosolic Ca is supported by the finding that the divalent cation ionophore A23187 also initiated 86Rb or K loss (as measured by an external K electrode). This increased K conductance was also seen with 10(-4) M ATP. Quinine and apamin greatly reduced the effect of ATP or A23187 on 86Rb or K release in adrenal glomerulosa cells. The results suggest that Ca-dependent K channels or carriers are present in the membranes of bovine adrenal glomerulosa cells and are sensitive to hormonal stimulus.

• Research Organization: Univ. of Chile, Santiago
• OSTI ID: 5529205
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
ALKALI METAL ISOTOPES
ALKALI METALS
ALKALINE EARTH ISOTOPES
ALKALOIDS
ANGIOTENSIN
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIMICROBIAL AGENTS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM 45
CALCIUM ISOTOPES
CARDIOVASCULAR AGENTS
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELEMENTS
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
GLOBULINS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KETONES
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
METALS
MINERALOCORTICOIDS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PERMEABILITY
POTASSIUM
PREGNANES
PROTEINS
QUININE
RADIOISOTOPES
RUBIDIUM 86
RUBIDIUM ISOTOPES
RUMINANTS
STEROID HORMONES
STEROIDS
STIMULATION
VASOCONSTRICTORS
VERTEBRATES