Effect of angiotensin II, ATP, and ionophore A23187 on potassium efflux in adrenal glomerulosa cells
Angiotensin II stimulus on perifused bovine adrenal glomerulosa cells elicited an increase in 86Rb efflux from cells previously equilibrated with the radioisotope. When 45Ca fluxes were measured under similar conditions, it was observed that Ca and Rb effluxes occurred within the first 30 s of the addition of the hormone and were independent of the presence of external Ca. The 86Rb efflux due to angiotensin II was inhibited by quinine and apamin. The hypothesis that the angiotensin II response is a consequence of an increase in the K permeability of the glomerulosa cell membrane triggered by an increase in cytosolic Ca is supported by the finding that the divalent cation ionophore A23187 also initiated 86Rb or K loss (as measured by an external K electrode). This increased K conductance was also seen with 10(-4) M ATP. Quinine and apamin greatly reduced the effect of ATP or A23187 on 86Rb or K release in adrenal glomerulosa cells. The results suggest that Ca-dependent K channels or carriers are present in the membranes of bovine adrenal glomerulosa cells and are sensitive to hormonal stimulus.
- Research Organization:
- Univ. of Chile, Santiago
- OSTI ID:
- 5529205
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States)
- Country of Publication:
- United States
- Language:
- English
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POTASSIUM
MEMBRANE TRANSPORT
ADRENAL GLANDS
ALDOSTERONE
ANGIOTENSIN
ATP
CALCIUM 45
CATTLE
CELL MEMBRANES
PERMEABILITY
QUININE
RUBIDIUM 86
STIMULATION
ADRENAL HORMONES
ALDEHYDES
ALKALI METAL ISOTOPES
ALKALI METALS
ALKALINE EARTH ISOTOPES
ALKALOIDS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIMICROBIAL AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELEMENTS
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
GLOBULINS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KETONES
MAMMALS
MEMBRANES
METALS
MINERALOCORTICOIDS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RADIOISOTOPES
RUBIDIUM ISOTOPES
RUMINANTS
STEROID HORMONES
STEROIDS
VASOCONSTRICTORS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques