Angiotensin effects on calcium and steroidogenesis in adrenal glomerulosa cells
We investigated the role of cellular calcium pools in angiotensin II-stimulated aldosterone synthesis in bovine adrenal glomerulosa cells. Angiotensin II decreased the size of the exchangeable cell calcium pool by 34%, consistent with previous observations that angiotensin II causes decreased uptake of /sup 45/Ca+2 into cells and increased efflux of /sup 45/Ca+2 from preloaded cells. Atomic absorption spectroscopy showed that angiotension II caused a decrease of 21% in total cellular calcium. Angiotensin II caused efflux of /sup 45/Ca+2 in the presence of EGTA and retarded uptake of /sup 45/Ca+2 when choline was substituted for sodium, suggesting that hormone effects on calcium pools do not involve influx of trigger calcium or sodium. Cells incubated in calcium-free buffer and 0.1 mM or 0.5 mM EGTA synthesized reduced (but still significant) amounts of the steroid in response to hormone. Cells incubated in increasing concentrations of extracellular calcium contained increasing amounts of intracellular calcium and synthesized increasing amounts of aldosterone in response to angiotensin II. These results point to the participation of intracellular calcium pools in angiotensin II-stimulated steroidogenesis and the importance of extracellular calcium in maintaining these pools.
- Research Organization:
- William S. Middleton Memorial Veterans Administration Hospital, Madison, WI
- OSTI ID:
- 5681130
- Journal Information:
- Endocrinology; (United States), Vol. 116:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ALDOSTERONE
BIOSYNTHESIS
ANGIOTENSIN
BIOLOGICAL FUNCTIONS
CALCIUM
ABSORPTION SPECTROSCOPY
ADRENAL GLANDS
CALCIUM 45
CELL CULTURES
CHOLINE
COWS
TRACER TECHNIQUES
ADRENAL HORMONES
ALCOHOLS
ALDEHYDES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CARDIOVASCULAR AGENTS
CATTLE
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELEMENTS
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
FUNCTIONS
GLANDS
GLOBULINS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIPOTROPIC FACTORS
MAMMALS
METALS
MINERALOCORTICOIDS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RUMINANTS
SPECTROSCOPY
STEROID HORMONES
STEROIDS
SYNTHESIS
VASOCONSTRICTORS
VERTEBRATES
550501* - Metabolism- Tracer Techniques