Energy and protein relations in the broiler chicken. 4. Role of sex, line and substrate on in vitro lipogenesis

Journal Article · · Growth; (United States)

OSTI ID:5519967

Rosebrough, R W; Steele, N C; McMurtry, J P; Richards, M P; Mitchell, A D; Calvert, C C

Experiments were conducted with dwarf (dw) and normal lines of chickens to determine the effect of sex, diet and line on lipogenesis in the 28-day-old chick. The chicks were fed diets containing 12, 18, 23 and 30% protein. In the first experiment, in vitro lipogenesis (incorporation of (2-/sup 14/C) sodium acetate into hepatic fatty acids) as well as growth from 7 to 28 days of age were determined in males and females of both lines. In the second experiment, only males and females of the dwarf line were fed to determine the relative contribution of acetate and pyruvate to in vitro lipogenesis (incorporation of either (2-/sup 14/C) sodium acetate or (2-/sup 14/C) pyruvate into hepatic fatty acids). Chicks of the dwarf line were smaller than were those of the normal line. Females of both lines were smaller than males. In vitro lipogenesis was lower in the dwarf line; however the rate for both sexes within a given line was equal. An increase in the dietary protein decreased in vitro lipogenesis in both lines. The use of pyruvate as an in vitro precursor indicated that the regulation of lipid and carbohydrate metabolism may be an integrated process involving pyruvate carboxylation and subsequent flux of pyruvate carbon into either glucose or fatty acids. Based on the data presented, there is no evidence to assume, that the dwarf gene per se influences lipogenesis.

• Research Organization: Dept. of Agriculture, Beltsville, MD
• OSTI ID: 5519967
• Journal Information: Growth; (United States), Journal Name: Growth; (United States) Vol. 50:4; ISSN GROWA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL VARIABILITY
BIRDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHICKENS
DIET
FOWL
GENES
GENETIC VARIABILITY
IN VITRO
ISOTOPE APPLICATIONS
KETO ACIDS
LABELLED COMPOUNDS
LIPIDS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
PYRUVIC ACID
SEX DEPENDENCE
SUBSTRATES
TRACER TECHNIQUES
VERTEBRATES