Lipogenesis and glucose production in dwarf carrier and normal lines of chicks

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5461378

Rosebrough, R W; McMurtry, J P; Steele, N C

Diets containing 13, 16, 19, or 23% protein and 70% carbohydrate calories were fed to dwarf heterozygote (dw) and normal (Dw) chickens to determine the effects of age (weeks) and protein on intermediary metabolism. In vitro lipogenesis (IVL) was determined by the incorporation of acetate (10 and 20 mM 2/sup 14/C-Acet/2hr) into hepatic fatty acids. Net glucose production (NGP) was determined as the difference in media glucose in the presence or absence of 10 mM pyruvate. Values were expressed per unit of relative liver size (..mu..moles/100 g BWt). Serum insulin (INS; ng/ml) was determined by homologous radioimmunoassay. Results indicate that although INS was greater in Dw than in dw, this difference was not reflected in a decreased rate of glucose production to accompany the difference in IVL between the two lines. Moreover, an increase in dietary protein resulted in a decrease in IVL but an increase in INS.

• Research Organization: Dept. of Agriculture, Beltsville, MD
• OSTI ID: 5461378
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BIOSYNTHESIS
BIRDS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHICKENS
DIET
DIGESTIVE SYSTEM
FOWL
GLANDS
GLUCOSE
HEXOSES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
METABOLISM
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SACCHARIDES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES