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Title: Interaction of dietary mercury and biotin-deficiency on hepatic lipogenesis in chicks

Abstract

Broilers were fed control or biotin-deficient diets based upon glucose and casein starting at day-old. At 7 days, one-half the chicks fed the biotin-deficient diet were administered 200 ppm Hg (as HgCl/sub 2/) continuously in the drinking water, while the remaining biotin-deficient chicks and the control chicks continued to receive tap water. At 4 weeks, hepatic fatty acid synthetse (FAS) and acetyl-CoA carboxylase (ACC) activities and in vivo lipogenesis from /sup 14/C-acetate were measured in randomly selected chicks from each of the 3 treatments. Specific activity of FAS was stimulated slightly by biotin-deficiency but Hg reduced activity to control values in deficient chicks. Specific activity of ACC was reduced to one-half of control values by biotin deficiency and Hg had no further effect. Incorporation of /sup 14/C-acetate into hepatic fatty acids was reduced markedly by biotin-deficiency but Hg returned incorporation to control values in deficient chicks. ACC is presumably the rate-limiting enzyme for lipogenesis. Therefore, the data suggest that Hg somehow stimulates the in vivo activity of this enzyme in biotin-deficient chicks.

Authors:
;
Publication Date:
Research Org.:
North Carolina State Univ., Raleigh
OSTI Identifier:
6300619
Resource Type:
Journal Article
Journal Name:
Poult. Sci.; (United States)
Additional Journal Information:
Journal Volume: 56:5
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ENZYMES; BIOSYNTHESIS; MERCURY; BIOLOGICAL EFFECTS; CARBON 14; CHICKENS; METABOLIC ACTIVATION; METABOLISM; STIMULATION; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIRDS; CARBON ISOTOPES; ELEMENTS; EVEN-EVEN NUCLEI; FOWL; ISOTOPES; LIGHT NUCLEI; METALS; NUCLEI; RADIOISOTOPES; SYNTHESIS; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)

Citation Formats

Hamilton, J C, and Donaldson, W E. Interaction of dietary mercury and biotin-deficiency on hepatic lipogenesis in chicks. United States: N. p., 1977. Web.
Hamilton, J C, & Donaldson, W E. Interaction of dietary mercury and biotin-deficiency on hepatic lipogenesis in chicks. United States.
Hamilton, J C, and Donaldson, W E. Sat . "Interaction of dietary mercury and biotin-deficiency on hepatic lipogenesis in chicks". United States.
@article{osti_6300619,
title = {Interaction of dietary mercury and biotin-deficiency on hepatic lipogenesis in chicks},
author = {Hamilton, J C and Donaldson, W E},
abstractNote = {Broilers were fed control or biotin-deficient diets based upon glucose and casein starting at day-old. At 7 days, one-half the chicks fed the biotin-deficient diet were administered 200 ppm Hg (as HgCl/sub 2/) continuously in the drinking water, while the remaining biotin-deficient chicks and the control chicks continued to receive tap water. At 4 weeks, hepatic fatty acid synthetse (FAS) and acetyl-CoA carboxylase (ACC) activities and in vivo lipogenesis from /sup 14/C-acetate were measured in randomly selected chicks from each of the 3 treatments. Specific activity of FAS was stimulated slightly by biotin-deficiency but Hg reduced activity to control values in deficient chicks. Specific activity of ACC was reduced to one-half of control values by biotin deficiency and Hg had no further effect. Incorporation of /sup 14/C-acetate into hepatic fatty acids was reduced markedly by biotin-deficiency but Hg returned incorporation to control values in deficient chicks. ACC is presumably the rate-limiting enzyme for lipogenesis. Therefore, the data suggest that Hg somehow stimulates the in vivo activity of this enzyme in biotin-deficient chicks.},
doi = {},
journal = {Poult. Sci.; (United States)},
number = ,
volume = 56:5,
place = {United States},
year = {1977},
month = {1}
}