Nonenzymatic glycosylation of human hemoglobin at multiple sites
Journal Article
·
· Metabolism; (United States)
The most abundant minor hemoglobin component of human hemolysate is Hb A1c, which has glucose bound to the N-terminus of the beta chain by a ketoamine linkage. Hb A1c is formed slowly and continuously throughout the 120 day lifespan of the red cell. It can be synthesized in vitro by incubating purified hemoglobin with 14C-glucose. Other minor components, Hb A1a1 and Hb A1a2 are adducts of sugar phosphates at the N-terminus of the beta chain. Hb A1b contains an unidentified nonphosphorylated sugar at the beta N-terminus. In addition, a significant portion of the major hemoglobin component (Hb Ao) is also glycosylated by a glucose ketoamine linkage at other sites on the molecule, including the N-terminus of the alpha chain and the epsilon-amino group of several lysine residues on both the alpha and the beta chains. The results indicate that the interaction of glucose and hemoglobin is rather nonspecific and suggests that other proteins are modified in a similar fashion.
- Research Organization:
- Hematology Division, Peter Bent Brigham Hospital, Harvard Medical School, Boston, MA
- OSTI ID:
- 5504394
- Journal Information:
- Metabolism; (United States), Journal Name: Metabolism; (United States) Vol. 28:4 Suppl 1; ISSN MTBMA
- Country of Publication:
- United States
- Language:
- English
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Effect of gamma irradiation on the. cap alpha. and. beta. chains of bovine hemoglobin and globin. [/sup 60/Co]
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5333028
ASSOCIATION OF RADIOACTIVE CHROMIUM WITH VARIOUS COMPONENTS OF HEMOGLOBIN
Journal Article
·
Mon Dec 31 23:00:00 EST 1962
· Blood (U.S.)
·
OSTI ID:4755897
Effect of gamma irradiation on the. cap alpha. and. beta. chains of bovine hemoglobin and globin. [/sup 60/Co]
Journal Article
·
Tue Mar 31 23:00:00 EST 1981
· Radiat. Res.; (United States)
·
OSTI ID:6538493
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
ERYTHROCYTES
GLOBIN
GLUCOSE
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MATERIALS
MOLECULAR STRUCTURE
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
REACTION KINETICS
SACCHARIDES
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
ERYTHROCYTES
GLOBIN
GLUCOSE
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MATERIALS
MOLECULAR STRUCTURE
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
REACTION KINETICS
SACCHARIDES