Effect of gamma irradiation on the. cap alpha. and. beta. chains of bovine hemoglobin and globin. [/sup 60/Co]
Hemoglobin was obtained from the blood of lowland black and white cattle with HbA phenotype. Water solutions of hemoglobin (Hb) or globin (Gl) in 20 mM KH/sub 2/PO/sub 4/ were ..gamma..-irradiated with a dose of 2 Mrad, and the amino acid composition of ..cap alpha.. and ..beta.. chains of control and irradiated Hb and Gl was analyzed. Quantitative differences were found between the radiation sensitivities of ..cap alpha.. and ..beta.. chains of Hb and Gl. A sequence of radiation sensitivity of individual amino acids in ..cap alpha.. and ..beta.. chains of Hb and Gl was determined. In the ..beta.. chains, amino acid destruction was considerably higher than in ..cap alpha.. chains. These changes were confirmed by amino acid analysis which showed that Cys, Met, Tyr, Arg, Ser, Thr, Pro, and His residues were most destroyed or modified following irradiation.
- Research Organization:
- Univ. of Lodz, Poland
- OSTI ID:
- 6538493
- Journal Information:
- Radiat. Res.; (United States), Vol. 86:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINO ACIDS
RADIOSENSITIVITY
GLOBIN
BIOLOGICAL RADIATION EFFECTS
HEMOGLOBIN
CATTLE
CHEMICAL COMPOSITION
COBALT 60
GAMMA RADIATION
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
COBALT ISOTOPES
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RUMINANTS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560114* - Radiation Effects on Biochemicals- In Animals- (-1987)
560152 - Radiation Effects on Animals- Animals